Is the Triple-Sugar Iron Agar (TSIA) a complex or defined medium? Explain based on its composition. Is the test tube a A) broth, B) slant, or C) deep agar medium? Why is a "needle" used to inoculate?

Q: What are the materials used to grow microorganism like Spirulina?

A: Introduction In this question we will discuss about the materials used to grow microorganisms like…

Q: Is  Bacillus cereus negative or positive for methyl red, and  Voges-Proskauer tests?

A: The methyl red (MR) test detects whether bacteria can ferment the glucose or not. It detects the…

Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…

A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…

Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…

A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…

Q: If an unknown bacteria was given that was gram-positive coccus, and the catalase test was performed,…

A: Gram-positive bacteria, such as Staphylococcus aureus, are bacteria that provide a positive response…

Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli

A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…

Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…

A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…

Q: Why is the level of contamination measured as number of colonies rather than size of colonies?

A: Microbiological contamination refers to the presence of unwanted or accidental introduction of…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: Is the Tryptoc Soy Agar (TSA) a complex defined medium? Explaining based on its composition?  Is TSA…

A: Trypticase soy agar  (TSA) and Trypticase soy broth is a growth media for the culturing of bacteria.

Q: Why would it be important for the Kirby-Bauer disc diffusion test to use a standard concentration of…

A: An antibiotic susceptibility test is the disk diffusion or agar diffusion test, or Kirby-Bauer test…

Q: If five colonies were counted on a 10–3 dilution plate streaked with 0.01 mlof urine, what was the…

A: The standard plate count estimates the total aerobic population density of microbes in a sample by…

Q: Which bacterium produced a yellow halo, and how do you interpret this result? •  If you grew…

A: Microbes require micronutrients and macronutrients for growth and multiplication. Required nutrients…

Q: Why are complex media preferable to chemically defined media for routinecultivation of…

A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…

Q: In the multiple fermentation tube technique does the absence of gas formation after 24 hours…

A: Coliform microbes are life forms that are available in the climate and in the defecation of all…

Q: According to the hemolysis results, how does Bacillus cereus differ from B. subtilis?

A: Given: To find how does Bacillus cereus differ from B. subtilis.

Q: Name five functional traits of microorganisms. What dose the functional diversity describe? Do we…

A: Functional traits of the microbes describe individually expressed phenotypes or characteristics of…

Q: Why is it important to use sterile practices when working with microorganisms?

A: Microscopic organisms such as bacteria, fungi (mold and yeast), protists, archaea, algae,…

Q: Why is it important to follow sterile technique?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: what is the culture media for gram-positive bacteria?

A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…

Q: What is the Microorganism involved in the spoilage of canned curdled evaporated milk and Glutinous…

A: Any microorganism attack can degrade the food product quality and make them non-consumable by…

Q: Which of the letter labeled microbes is exhibiting alpha-hemolysis?

A: The lysis or breakdown of red blood cells is called hemolysis. There are three types of hemolysis…

Q: Is the autoclaving process absolute in killing all microorganisms? If not, what are the factors that…

A: Autoclave is an instrument or device which is used in  method of moist heat sterilisation; while…

Q: Media is TGA explaining based on what kind of microorganisms it allows to grow?

A: TGA is a complex media based upon its chemical composition. Complex media are the media in which the…

Q: What is selective medium?

A: Description of a selective medium.

Q: Why are the streak plates incubated at 7°C when selecting for psychrotrophic bacteria?

A: Bacteria are unicellular, prokaryotic organisms which are devoid of membrane bound organelles and a…

Q: why are psychrotrophic bacteria incubated at 7 degrees

A: Each species of microbe has a preferred temperature that lies somewhere between its characteristic…

Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?

A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…

Q: Is the pH level of antibacterial soap higher, lower, or the same as H. influenzae, S. aureus and S.…

A: Antibacterial soap These are the soaps which have chemical ingredients that have the ability to…

Q: Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is…

A: Mueller and Hinton in 1941 developed Mueller Hinton Agar(MHA). MHA is used for routine…

Q: Before using the API® Staph System, what tests should be performed on bacteria to confirm that they…

A: API Staph (analytical profile index) is a systematized procedure used for the identification of the…

Q: What is the optimum growth temperature for most human pathogens? Explain.

A: Bacterial growth is the division of one bacterium into two daughter cells in a process called binary…

Q: Agar is a complex polysaccharide obtained from seaweed and cannot be digested by most…

A: Growth media is the nutrients rich medium that helps in the growth of several bacteria and other…

Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…

A: Bacteria are of many types. They can be classified based on different properties such as cell wall…

Q: Why do we need to transport the throat swab using transport culture medium?

A: Transport media are essentially solutions of buffers with carbohydrates, peptones and other…

Q: Describe what three strategies are used to identify microorganisms?

A: Microorganisms: Microorganisms are tiny organisms that are not visible through an unaided eye.…

Q: What is  bactericide exposure?

A: The term bactericide is related to bacteria. Bacteria are unicellular and prokaryotic organisms that…

Q: Could humans exist on chemically defined media for 48 hours?

A: A chemically defined medium is a growth medium whose components and their concentration (or…

Q: Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies…

A: Urine collection for the urinary tract infection.

Q: 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and…

A: The culture medium is a solid, semi-solid or liquid substrate that is rich in nutrients and other…

Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…

A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…

Q: Which bacterium showed some hydrogen sulfide precipitate after 24 hours? Would you expect this to…

A: The TSIA test is an acronym for triple sugar iron agar test which is used to determine the…

Q: why is Brocardia anammoxidan a Gram-negative bacteria? what does gram negative bacteria mean?

A: Answer. Bacteria are microscopic, relatively simple, prokaryotic organisms whose cell lacks a…

Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…

A: Introduction KIA or the Kilger Iron Agar is a differential media that helps in distinguishing the…

Q: How is the CTSA (coconut tryptic soy agar plate) growth medium designed to meet the nutritional need…

A: TSA is a general-purpose plating medium that can be used to isolate, cultivate, and maintain a wide…

Q: Why do petroleum-degrading bacteria need to attach to thesurface of oil droplets?

A: Petroleum is a crucial strategic resource and all countries compete fiercely for it. The…

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…

A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…