Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: what is the procedure and materials you need to stain Staphylococcus aureus?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…
Q: what is the importance of getting retention factor (rf) value in chromotography tools?
A: Rf also known as retention factor is used in thin layer chromatography (TLC) and high performance…
Q: explain why it is not good to break sterile field when inserting a foley catheter
A: Inserting the indwelling catheter is an invasive procedure. Sterile technique should follow while…
Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…
A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…
Q: The ___________ color test reagent turns orange-brownin the presence of amphetamines.
A: A biochemical substance that induces physiological responses, when introduced into the body, is the…
Q: Why might Gamma irradiation be considered better than using UV radiation?
A: It is required to provide reason that Gamma irradiation is considered better than using UV radiation…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: How are clinical chemistry test results used to confirm or rule out a presumptive diagnosis? Why is…
A: Any medical test's primary goal is to assess if a patient does or does not have a specific disorder.
Q: What pathogenic agent undergoes the Sigmoidoscopy Material procedure?
A: The sigmaidoscopy procedure test uses a thin flexible tube with a camera and light on one end. The…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: Which objective is referred to as the "scanning objective"? 4x 10x 20x 100x 400x
A: The least powerful lens is called the scanning objective lens and is typically a 4X objective. A…
Q: Explain the usage of photoactivated localization microscopy technique.
A: Photoactivated localization microscopy abbreviated as PALM is a microscopy technique that was…
Q: Application of differential staining technique.
A: Differential staining is a staining technique that employs the application of several different…
Q: Preservatives are added to the following pharmaceutical products EXCEPT Select one: Ophthalmic…
A: Any substances that are added to the foods and pharmaceutical products to prolong its shelf life…
Q: Why is the accuracy of the calibration curve so critical?
A: Calibration curves are the graphs showing how analytical or instrumental response changes with the…
Q: Why is UV light used in Column chromatography experiment ?
A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…
Q: g technique, and what is the target organisms and the purpose of its application? LONG ANSWER…
A: The bacterial staining can be simple as well as differentiated. It is studied under the domain of…
Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.
A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…
Q: What are the possible sources of contamination that may result during the streak plate procedure?
A: The streak plate procedure is used to isolate microbial colonies in pure form. During the procedure,…
Q: Why is it necessary to use immersion oil when the 100X objective is used'
A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…
Q: Discuss sabin feldman dye test.
A: A Sabin–Feldman dye test is a serologic test for toxoplasmosis diagnosis. Toxoplasma trophozoites…
Q: The following figures are the photomicrographs of the samples taken with Kern&Sohn Camera Microscope…
A: Ans: Lugols solution: The Lugols solution is 2.5 to 25% weight by volume total iodine.
Q: Discuss the significance of the following processes and what constituent is being determined: a.…
A: Borntrager’s Test: This is a quantitative chemical test for the glycosides in any given sample, this…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: What is the importance of performing the Modified Allen Test? Give at least three.
A: Modified Allen test: This test is done to assess the integrity of the ulnar artery, To perform this…
Q: Sources of error and precautions when doing the creatinine test using the Jaffe Method
A: Creatinine is the degraded product of creatine and phosphocreatine, which is used to test the renal…
Q: What color tube is used for creatinine test?
A: Introduction:- Creatinine test is done to assess the situation of the kidney. Creatinine values…
Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…
A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…
Q: Why are specimens for bilirubin testing protected from light? Discuss handling procedures for…
A: Bilirubin is a yellow pigment that is present in bile. It is formed as a result of breakdown of red…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: A photo-multiplier tube converts light into x-rays. True False
A: 1. ANS -TRUE, PHOTON MULTIPLE TUBE ( PMT) CONVERT LIGHT TO X RAY . A photomultiplier tube (PMT)…
Q: True or False: Ultrathin sections are used for light microscopy but are to thin to be stable under…
A: Microscopes are used to visualize objects that are not visible to the naked eye. In microbiology,…
Q: The most commonly employed chemical test to determine the presence of blood in stain found at the…
A: Answer is option E"Teichman's test". this test is also called by names haematin crystal acid test or…
Q: What tests are included in a CBC? Give the normal value,and name a disorder that would cause an…
A: Complete blood test It is the test to perform to evaluate various parameters of blood cells. Purpose…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: If, when using 10X ocular and a 20X objective, the field size is 2 mm. The approximate field size…
A: Ocular magnification - This is the magnification that can be viewed by the ocular lenses, the lenses…
Q: What advantage does the oil immersion objective have over the low power objective when doing…
A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Write down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
A: It is a differential staining technique which was first developed by Ziehl and later on modified by…
Q: The Duquenois-Levine test is a valuable color test for
A: We can say that, In a forensic chemical analysis of an unknown material, the analytical method is to…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: In RPR test, Why is it necessary to rotate the slide/card?
A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.
Q: Why rapid tests are not the gold standard in Covid19 detection. Explain.
A: Diagnosis of the Sar-Cov-2 virus is usually done by means of isolating samples collected from the…
Q: The Duquenois–Levine test is a valuable color test for___________.
A: In a forensic chemical analysis of an unknown material, the analytical method is to narrow down the…
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- What are the principle and basic concepts of NEGATIVE staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)What are the principle and basic concepts of DIFFERENTIAL staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
