Give three situations where the negative staining procedure would be used.

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: is it essential that the primary stain and counterstain be of contrasting colors

A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…

Q: Define fluorescent staining.

A: The advent of microscopy started with the development of optical microscopy. Then the level of…

Q: Crystal violet is an example of what type of stain?

A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Give two reasons for heating the slide after the smear is air dried?

A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…

Q: Why is there a need to use a staining agent in observing the specimen?

A: Staining agent:          The things that owes-out stains were recognized as staining agent,…

Q: Differentiate between negative and positive staining, givingexamples.

A: These are the staining methods that are used to see bacteria under a microscope.

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…

Q: List the various staining methods, and briefly characterize each.

A: Regardless of the ability of the microscope to magnify and resolve, staining is necessary to create…

Q: Give three situations where negative staing procedure would be used?

A: Negative staining is a staining technique in which the background is stained whereas the specimen…

Q: Give the principle of acid fast staining and gram staining.

A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…

Q: Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining…

A: The correct option is . A Gram's Staining procedure

Q: What are the functions of a primary stain and a counterstain?

A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…

Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…

A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: staining

A: Staining-  It is used to enhance contrast in samples, generally at the microscopic level. In other…

Q: What is an example of a negative stain?

A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…

Q: A concept map for staining technique.

A:

Q: Compare and contrast simple, differential, and structural stains, and give examples for each.

A: Introduction Staining Is A Technique For Enhancing Contrast In Specimens, Usually On A Microscopic…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: Differentiate between simple and differential stains

A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Q: In negative staining, the acidic stain will penetrate the cell. In negative staining, it will create…

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Why is it necessary to use contrasting stains while staining

A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: Briefly answer the question below: What is the disadvantage of having a really thick smear when…

A: Smear:- It is used to fix the bacteria onto the slide and to prevent the sample from being lost…

Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…

A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…

Q: Positive Staining Negative staining

A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…

Q: The primary stain used in the endospore stain is:    A. Carbolfuchsin Stain B. Methylene  Blue C.…

A: To differentiate between the vegetative cells and the endospores, a differential staining technique…

Q: Give two factors that may affect a good gram staining technique output. Provide an explanation how…

A: Many factors can affect the results of gram staining. However, two important factors that may affect…

Q: Define dyes and describe the basic chemistry behind the processof staining.

A: Detection and observation of cells and microorganisms in their natural state under microscope is…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: Explain the difference between resolution and mag­nification.

A: Introduction :- The term'resolution' is used in microscopy to describe a microscope's ability to…

Q: In staining, why is there a need for contrasting stains?

A: Answer

Q: Draw one acceptable pattern that is used in streak plate method

A: Streak plate method : It is used for the isolation into a pure culture of the organisms , f9om a…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: k there are 2 staining reagents applied in differential s

A: Differential Staining-  It is a staining process which uses more than one staining reagent.  it is…

Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…

A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…

Q: differential staining procedures

A: Differential staining :  Differential staining is one of the important staining method. In this…

Q: How is staining techniques useful in the clinical settings

A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…

Q: stain

A: Cartilage is a smooth elastic tissue, it protects the bones at the joint by padding it.

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…

Q: The Duquenois–Levine test is a valuable color test for___________.

A: In a forensic chemical analysis of an unknown material, the analytical method is to narrow down the…

Q: Name two conditions, which will affect the quality of your staining. B.What is a panoptic stain?…

A: Staining is a very important part in Histology, without which it would be impossible to study the…

Q: cold method of Acid-fast staining.

A: