Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process?

Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 1CT

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Question

Why are basic dyes more effective for bacterial staining than acidic dyes?
State two ways that can confirm whether a bacterial smear has been correctly prepared or not.
Why should you be careful not to underheat a smear during the heat-fixing process?
Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.

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