Tris or tris(hydroxymethyl)aminomethane is an organic buffering reagent most often used in nucleic acid extractions. Tris (MW = 121.14 g/mol) has a pKa of 8.07 at 25oC, with a white crystalline powder appearance. What is the buffering range/s of Tris buffer? How would you prepare 500mL of a 0.200 M Tris buffer solution using Tris and NaTris or sodium Tris (MW = 143.14 g/mol)?
Tris or tris(hydroxymethyl)aminomethane is an organic buffering reagent most often used in nucleic acid extractions. Tris (MW = 121.14 g/mol) has a pKa of 8.07 at 25oC, with a white crystalline powder appearance. What is the buffering range/s of Tris buffer? How would you prepare 500mL of a 0.200 M Tris buffer solution using Tris and NaTris or sodium Tris (MW = 143.14 g/mol)?
Chapter16: Applications Of Neutralization Titrations
Section: Chapter Questions
Problem 16.20QAP
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Tris or tris(hydroxymethyl)aminomethane is an organic buffering reagent most often
used in
with a white crystalline powder appearance. What is the buffering range/s of Tris buffer?
How would you prepare 500mL of a 0.200 M Tris buffer solution using Tris and NaTris or
sodium Tris (MW = 143.14 g/mol)?
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