Answered: (a) From the information given in the… | bartleby

Case Studies In Health Information Management

Case Studies In Health Information Management

3rd Edition

ISBN:9781337676908

Author:SCHNERING

Publisher:SCHNERING

Chapter6: Leadership

Section: Chapter Questions

Problem 6.18.3C

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PROBLEM 2: A biochemist dliscovers and purifies a new enzyme, generating a purification table
below
The step of
protein
purification
Total
Total
Specific
Total
The multi-
Total
activity
yicld
(%)
cnzyme
fold
protcin
(mg)
maltifold
activity
increase in
purification
(units)
purity
Whole-cell lysate
8.4
LO
500
l00
4200
40% ammonium
22.2
95
2.64
9.64
180
3990
sulfate
Ion exchange
90.2
251
6.63
68
3787
55.7
Gel filtration
50
5000
to0
119
1.8
11.9
(a) From the information given in the table, calculate the specific activity of the enzyme,
total yield (%), multi-fold increase in purity, and total multifold purification after each
purification procedure.
b) Which of the purification procedures used for the enzyme is most effective (Le., gives
the greatest relative increase in purity)?
(c) Which step - ion-exchange or gel filtration chromatography - causes the greatest loss
of the protein.
(d) If you wanted to use only one purification step, which technique would
you
choose?

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