For the following aspartase reaction in the presence of the inhibitor hydroxymethylaspartate, determine Km and whether the inhibition is competitive or noncompetitive. You have to plot the graph on the graph paper and also by using excel. [S] V, No Inhibitor V, Inhibitor Present (molarity) (arbitrary units) (same arbitrary units) 1 x 10-4 0.026 0.010 5 x 10-4 0.092 0.040 1.5 x 10-3 0.136 0.086 2.5 x 10-3 0.150 0.120 5 x 10-3 0.165 0.142

For the following aspartase reaction in the presence of the inhibitor hydroxymethylaspartate, determine Km and whether the inhibition is competitive or noncompetitive. You have to plot the graph on the graph paper and also by using excel. [S] V, No Inhibitor V, Inhibitor Present (molarity) (arbitrary units) (same arbitrary units) 1 x 10-4 0.026 0.010 5 x 10-4 0.092 0.040 1.5 x 10-3 0.136 0.086 2.5 x 10-3 0.150 0.120 5 x 10-3 0.165 0.142

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter25: Nitrogen Acquisition And Amino Acid Metabolism

Section: Chapter Questions

Problem 22P

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