When using a light microscope, objective lenses can be found to have a magnification of all of the following, EXCEPT? A 4X в 10х C 40X D 100X 1000X
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A: Introduction An instrument used to magnify small objects called a microscope. Even at the cellular…
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Q: You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following…
A: Objective lens The objectives lens have various magnification powers with common values of 4x, 10x,…
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A: An optical microscope is another name for a light microscope.
Q: Why should you always begin to use a microscope with a low-power objective?
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Q: focusing
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Q: ou have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following…
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A: given that the initial two magnifications are 5x and 10x , and final magnification is 5x and 40x
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A: A compound microscope has multiple lenses of different magnification.
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Q: Non-conducting materials can be studied by scanning tunneling microscope(STM). 1.True 2. False
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Q: 5 1 2 6. 4 7 8.
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Q: the vacuum is very important stage during electron microscope operation O true O False
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- In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the application of decolorizing agent, it leaves the acid fast cells colorless. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.write a short not on insoluble polymer matrix system of modified dosage form. question is related to pharmaceutical subject so this question accordingly.Please answer all parts as they are related to each other 1. Cells naturally have a negative charge. What are some compounds that give cells this negative charge? 2. We wash away the dye when we make a positive stain, but not when we make a negative stain. a. What would you see in your microscope if you made a positive stain and did not wash away the dye? b. What would you see in your microscope if you made a negative stain and did wash away the dye? 3. Draw an example of what you might expect to see under the microscope if you were looking at the following microbes. Use the genus name to determine cell shape and arrangement. Remember that positive and negative stains look different as well! a. Negative stain of Diplococcus pneumoniae b. Positive stain of Streptobacilus ratti
- Fluorescence intensities depends on the molecular structure and its environment. Discuss the factors that can affect the fluorescence intensity and explain how the factors increase or decrease the intensity.Hoechst 33342 is a membrane-permeant dye thatfluoresces when it binds to DNA. When a population ofcells is incubated briefly with Hoechst dye and then sortedin a flow cytometer, which measures the fluorescence ofeach cell, the cells display various levels of fluorescence asshown in Figure Q17–1.In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nm
- Eosin (an acidic dye, negatively charged) is used to stain cytoplasm (basic, negatively charged), and at the same time, Methylene blue (a basic dye, positively charged) can also be used to stain cytoplasm (basic, negatively charged). Why both acidic and basic dye can be used to stain cytoplasm? Why the negative charged Eosin can also bind to negative charged cytoplasm without repulsion?Suppose you have a suspension of HeLa cells with a cell count of 85,000 cellsper ml, and you want to prepare a suspension for your experiment that has a cellcount of 40,000 cells per ml. You need 10 mls total for your experiment. Howmany mls of cell suspension will you add to your experimental tube? How manymls of cell culture media to bring the volume up to 10 mls?Volume of cell suspension: _____________________________________Volume of cell culture media:____Assume the diameter of the field of vision in your microscope is 1.75mm under low pressure. If one bacillus is 5um, how many bacillus cell could fit end to end across the field. How many 7um yeast cells could fit across the field?
- In histopathology, why do different stains have different affinities for different organisms or different parts of organisms? Explain and include the Hematoxylin and Eosin staining as an example to further understand. Please answer the question concisely and don't just simply give the meaning of Hematoxylin and Eosin staining.Can the Eukaryotic Cell (Animal) shape enclosed in the circle with all the shape's markings be drawn by hand? Note: The drawing should look like a student drawing and not an expert drawing, please make the marking on the drawing complete and in a clear line away from mixing wordsDifferent stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.