Using the TABLE shown, identify which proteins would elute together in a gel filtration experiment. 1 & 2 and 4 & 5 1, 2 & 4 2 & 3 1 & 2 4 & 5

Biology (MindTap Course List)
11th Edition
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Chapter3: The Chemistry Of Life: Organic Compounds
Section3.1: Carbon Atoms And Organic Molecules
Problem 3C
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Using the TABLE shown, identify which proteins would elute together in a gel filtration experiment.
1 & 2 and 4 & 5
1, 2 & 4
2 & 3
1 & 2
4 & 5
Transcribed Image Text:Using the TABLE shown, identify which proteins would elute together in a gel filtration experiment. 1 & 2 and 4 & 5 1, 2 & 4 2 & 3 1 & 2 4 & 5
Net charge Number of Identical Disulfide
subunits
Protein
Molecular
Weight
at pH 7
subunits bonds
1
50,000
+3
1
No
50,000 (25,000 for
each subunit)
2
-2
2
Yes
No
25,000
Yes
100,000 (50,000
for each subunit)
4
-1
2
No
Yes,
between
subunits
100,000 (25,000
for each subunit)
5
+2
4
No
Yes,
between all
subunits
Transcribed Image Text:Net charge Number of Identical Disulfide subunits Protein Molecular Weight at pH 7 subunits bonds 1 50,000 +3 1 No 50,000 (25,000 for each subunit) 2 -2 2 Yes No 25,000 Yes 100,000 (50,000 for each subunit) 4 -1 2 No Yes, between subunits 100,000 (25,000 for each subunit) 5 +2 4 No Yes, between all subunits
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