Using the sodium chloride equivalent method, make the following solution isotonic with respect to the mucous lining of the eye (ocular membrane): Tetracycline hydrochloride: 10 g NaCl: x g Sterile distilled water, enough to make 1000 mL Report your answer in grams (to one decimal place).
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- Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.Given a stock protein solution with a concentration of 6 mg/ml, determine the protein concentration of a solution made by mixing 5 μl of the stock with 5 μl of a buffer.If you had measured the absorbances of your unknown in test tubes that were half the diameter of the ones that you used to make the standard curve, how would the mg of protein in the samples be affected? (Higher, Lower, No Change)
- What might happen if you omit potassium tellurite in using GCM?A glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equationWhich of the following options shows the correct order of fastest to slowest motion of chloride ions, glycine and proteins through the separating gel? Chloride ions > Proteins > Glycine Glycine > Chloride ions > Proteins Chloride ions > Glycine > Protein Protein > Chloride ions > Glycine
- Compute for the sodium concentration of the patient. Use the same formula in the computation of the concentration using absorbance and concentration of the standard. Given: Standard sample Concentration : 150mmol/L Absorbance: 0.112 Patient's sample Absorbance : 0.226 Concentration : ??Rx Ephedrine sulfate (fz. pt = -0.13°C). 2%Sodium chloride MW 58.5Purified water qs ad. 30 mL How much sodium chloride should be used to make this eye solution isotonic with tears? the answer is 224mgTable 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
- for 0.9% NaCl solution and 10% NaCl solution, which one has higher concentration of WATER?List the five components of an isoelectric focussing gelSolution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…