Compute for the lactic acid content of these samples given the values below. Show your solution Volume of 0.1 N NaOH in 1st Sampling: 10 mL Volume of 0.1 N NaOH in 2nd Sampling: 40 mL Volume of sample used: 10ml
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Compute for the lactic acid content of these samples given the values below. Show your solution
Volume of 0.1 N NaOH in 1st Sampling: 10 mL
Volume of 0.1 N NaOH in 2nd Sampling: 40 mL
Volume of sample used: 10ml
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- You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.topic: Determination of Protein Concentration by Spectrophotometry Enumerate and discuss the limitations of Beer-Lambert’s Law.Calculate how much agarose is needed to make a 3% agarose gel in a volume of 150 ml 1x TAE buffer.
- Computation: Ratio Strength, PPM, mg%Show your complete solution.1. How many grams of a 1:80 trituration of strychnine sulfate should be used for this prescription?RxStrychnine sulfate 45mgBelladonna extract 360mgSucrose 36gFt. chart; Div. in #XXXVIAmount of Strychnine in grams = ?please do not give solution in image format thanku Refer to the graph below, showing surface area and volume as functions of body size. Because of the relationships shown in the graphsuctose density gradient ultractifugation is a powerful technique for fractionating macromolecules like DNA,RNA and proteins.some protocols using sucrose gradients mention the following:10 mL sucrose gradients 10-15%(w/v) in 10 mM HEPES buffer.How would you prepare this sucros gradient?
- The protein concentration of a known standard is 100mg/mL If you prepared a serial dilution, mixing 10μL of protein with 40μL of water what would be concentrations of the first 3 dilutions?give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.Given the following information, calculate the total activity in the undiluted protein sample. Activity of 1 ml of diluted sample = 0.5 Total volume of sample = 5 ml Dilution factor = 10 25 50.5 250 2.5
- When comparing the biuret reaction to measuring protein absorbance directly at 280 nm, list two benefits of utilizing the biuret reaction to quantify protein concentration.i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).give the respective the TOTAL DILUTION FACTOR of respective serial dilutions. show complete solution.