What advantage do alternative sigma factors have for bacterial gene expression?
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- How does the sigma factor control whether it can start transcription together to give way to RNA polymerase take place on the sequence? Explain briefly.To understand the genes responsible for growth and infectivity in a disease-causing bacterial strain, you perform chemical mutagenesis on a culture of these bacteria. In the course of your investigation into the properties of the resulting mutants, you identify a set of mutant bacteria that is still viable but their virulence is significantly impaired. How might these mutants be useful for vaccine development?Can you explain in technical detail how alternative sigma factors and quorum sensing work and what they are used for? Thank You
- In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan.What is the primary function of the sigma factor? Is therea protein in eukaryotes analogous to the sigma factor?Given the following genotypes, explain, by answering the questions in each number, how the mutation (identified by a (-) superscript) will affect E. coli grown in lactose medium. Will there be a complete set ofgene products? (Yes/No) Will the lac operon be turnedon/off? Will the cell survive? (Yes/No) a. i + p + o + z - y + b. i + p - o + z + y + c. i + p + o - z + y +
- The consensus sequence is 5'TTGACA3' at –35 of bacterial promoters. Which of the following would be bound most strongly by sigma factor?Question 13 options: 5'AACTGA3' 5'TAAATT3' 5'TTCAAA3' 5'TTGAAA3'When Avery and his colleagues had obtained what was concluded to be the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, RNase, and DNase, followed in each case by the assay for retention or loss of transforming ability. What were the purpose and results of these experiments? What conclusions were drawn?"CRISPR-Cas Is an Adaptive Immune System in Bacteria" Explain this ?