In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 µmoles when the A+T/G+C ratio is 6.0. How much cytosine should be in the sample? A 4 3.5 2
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A: Dna is made from nitrogenous bases in which adenine binds to thymine Guanine binds to cytosine
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A: A DNA is composed of basic 4 nucleotide bases: Adenine (A) Guanine (G) Cytosine (C) Thymine (T)
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Q: In a sample dsDNA from an organism, the amount of thymine is analyzed to be 12 μmoles when the…
A: In double stranded DNA the composition of bases follows the rule: A+T= G+C.
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Q: Given the following ssDNA, what would be the complementary strand for it to be dsDNA?…
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A: Answer - option D.
Q: How many base pairs are there in a 6.8 nanometer DNA sequence? A. 5 B. 10 C. 15 D. 20
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Q: One OD unit of A260 is 50 ug/ml dsDNA. You have 2.75 ml genomic DNA (stock). You took 5C ul of the…
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- You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionAssume you have an SAR of 0.206 for sample 1; 0.271 for sample 2; and 0.606 for sample 3, what does this indicate? A larger increase in the purity of phycocyanin is seen after the use of ammonium sulphate, which removes other proteins A larger increase in the purity of phycocyanin is seen after the use of polyethylenimine, which removes other proteins A larger increase in the purity of phycocyanin is seen after the use of polyethylenimine, which removes nucleotides A larger increase in the purity of phycocyanin is seen after the use of ammonium sulphate, which removes nucleotidesCan someone explain why those values were chosen to be divided by the total? I thought it was the four smallest amounts, so I would have done 3+64+67++7/1600. Can someone explain why that would have been wrong
- You have a 2.5 ng/uL DNA stock. What volume will give you 1.15 mg?According to Chargaff’s rules for DNA base composition, if a DNA sample contained 10% guanine, the sample should have: A. 40% cytosine B. 90% cytosine C. 10% adenine D. 40% thymineExplain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.
- TRUE or FALSE a) The deoxyribose connects to N9 of guanine. b) In a dsDNA, a pyrimidine in one chain is always paired with a purine on the other chain because of this arrangement, the molecule is 2nm wide along its entire length. Example is A with G or C with T.What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.Assume that the molar percentage of thymine in a double stranded DNA is 20. What are the percentages of the four bases (G, C, T, A)? b. The base content of a sample of DNA is as follows: A=31% G=31% T=19% C=19%. What conclusion can be drawn from this information?
- 1 paragraph write-up that compares and contrasts Punnett Squares and the Hardy-Weinberg EquationIn primer designing, which of the following statements is correct? a. Primers should be 18-24 bases in length. b. Base composition should be 45-55% (G+C). c. Melting temperatures between 55-70°C are preferred. d. All choices are correct.The letter R isconventionally used to represent anypurine base (A or G) and Y to represent anypyrimidine base (T or C). In double-strandedDNA, what is the relation between %Rand %Y?
