how color relate to the wavelength of light absorbed by solution and lambda max?
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- Why are quantitative absorbance measurements made at lambda max?What is the absorption peak(s) of methyl orange? Make sure to write down the wavelength at which the absorption peak(s) occur and include units What color(s) does methyl orange absorb the best?calculate Evenness index, Simpson index, and Shannon-Weiner index
- What is the absorption peak(s) of methyl orange? Make sure to write down the wavelength at which the absorption peak(s) occur. Include units, please What color(s) does methyl orange absorb the best?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.Explain the limitations of the following expressions: (a) DS = C ln(Tf /Ti), (b) DG = DH − TDS, and (c) DG = wmax,non-exp.
- Assume that you have an “X” solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. Prepare a standard curve by using the absorbance of known concentrations (Hint: you can learn how to prepare a standard curve by using Excell from youtube). Calculate the concentration of unknown.Suppose you conduct direct visible spectrophotometry with red fruit punch as your solution. Which wavelengths of light would you expect to be strongly absorbed? Which wavelengths are not strongly absorbed? Explain.Using the data in this table, what is the AG° (in KJ/mol) for the reduction of FAD by water?