What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?

Q: Illustrate the step by step method of preparing culture media.

A: Introduction Culture medium:- It is a liquid or gel designed for specific mixtures of nutrients and…

Q: hat is the advantage of culture-independent analysis over culture-dependent analysis of…

A: An unknown microorganism can be identified and classified in a variety of ways. They are divided…

Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: What makes a culture media DIFFERENTIAL? Explain and provide examples of differential type of…

A: Answer: Culture media is the growth medium or culture medium which is solid, liquid, or semisolid in…

Q: In what instances would the pour plate method be more appropriate than the spread plat method?

A: Pour plate method is a technique that used to enumerate the number of viable cells in a sample.…

Q: List the two main types of culture media and how each differs and the types of microbes which grow…

A: Culture media contains all the elements which the bacteria needs for growth and is not selective in…

Q: Identify one culture-based and one non-culture-based method used for analyzing bacterial growth.

A: Bacteria divide by binary fission. They grow in an exponential manner. Four different phases can be…

Q: What order should the steps be in for this culture method?

A: A conditional mutant is a mutant that exhibits a wild-type phenotype under permissive conditions but…

Q: What are the 4 types of culture?

A: Biological cultures include common laboratory means, which are involved in studying living organisms…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: how is the streak plate technique used to isolate and purify psychrotrophic spoilage-causing…

A: Microbiology is an indispensable subject of biological sciences that concerns the study of microbes…

Q: What is the application of differential centrifugation?

A: In the laboratories, different kinds of techniques and devices are used to separate components of a…

Q: describe the agar-overlay technique

A: Biological techniques are processes or methods for trying to study living things. They include…

Q: What should be taken into consideration in the selection of the culture preservation method?

A: The problem of maintaining microbial culture is one that many fields of microbiology face. The…

Q: Why are complex media preferable to chemically defined media for routinecultivation of…

A: Microorganisms require nutrients as an energy source and certain other environmental conditions to…

Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

Q: What are the purposes of carrying out the streak plate andpour plate techniques in microbiology?

A: It is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: What are the factors which need to be considered in the selection of culture vessel?

A: Culture vessels:- Culture vessels provide a contamination barrier to protect the cultures from the…

Q: What makes a culture media as SELECTIVE? Explain and provide examples of selective type of culture…

A: A culture medium or growth medium is a substance that is used to grow the microorganisms under…

Q: Name five functional traits of microorganisms. What dose the functional diversity describe? Do we…

A: Functional traits of the microbes describe individually expressed phenotypes or characteristics of…

Q: what is the culture media for gram-positive bacteria?

A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: What is the basis of enrichment culture techniques? Why is an enrichment medium usually suitable for…

A: Enrichment culture media is the one which is used for the growth and isolation of a specific type of…

Q: What is the importance of using a test/control organism in describing the cultural characteristics…

A: The food materials on which the organism is developed is called culture medium and the development…

Q: What is a pure culture and how can one be obtained

A: Cells are the structural and functional units of life.

Q: How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: What is an alternative to autoclaving for sterilizing heat-sensitive materials?

A: Any process that kills, eliminates, deactivates, or removes all forms of microorganisms and…

Q: What is the difference between in-situ & ex-situ conservation?

A: Conservation plays a major role in preserving animal and plant species that are in the verge of…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: Can culture media be sterilized in the hot air oven? why or why not?

A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…

Q: What is a complex medium? and What are the basic components of a culture medium?

A: Medium is defined as the solid or liquid substratum on which the cells or organ can grow for further…

Q: Distinguish batch culture and continuous culture

A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…

Q: How can moist heat sterilization can sterilize antibiotic solution?

A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…

Q: What compounds or chemicals might be added to a medium to make it selective?

A: Culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth of…

Q: Can simply observing the colonies formed on agar plates and the results of a few biochemical tests…

A: Bacteria from colonies in the agar plates and different bacterial colonies will differ in shape,…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…

Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…

A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: What are the culture media that can be used for cultivating non-specific and specific…

A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…

Q: How do we isolate culture media (step by step process) and what is the purpose of bacterial culture…

A: Introduction :- Microorganisms can be found in the natural environment, such as soil. They coexist…

Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…

A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…

Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…

A:

Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.

A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Q: Are there technologies that can help make Bacterial Culture and Sensitivity done faster?

A: Microbial culture refers to the culture or growing the bacteria in the laboratories on liquid and…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.