Q: What is the difference of washing from hand disinfection?
A: Introduction:- Hand hygiene is very important for health. Hands are very important route of germs…
Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: Explain how to correctly use the microscope using oil immersion.
A: Light tends to bend while entering one material from a different one. The refraction of light…
Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *
A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…
Q: what does chroomatography observation mean
A: Column fractionation was developed in the 1890s as a technique of separating the various components…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.
A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…
Q: How would you determine the contents of the test tubes if all of the samples were transparent?
A: The study of chemical reactions that occurs within living organisms is called biochemistry. This…
Q: What is a pure culture?
A: Introduction Culture is referred to th process of growing the cell outside the animal or plant body…
Q: Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe…
A: 1. Glass slides on which the sample is placed. 2. Cover slip made up of plastic. 3. Dropper 4.…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate
A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…
Q: What a scrubber in the exhaust of chemical industrial plant removes?
A: Pollution is the introduction of contaminants into the natural environment that cause adverse…
Q: What are the 4 types of culture?
A: Biological cultures include common laboratory means, which are involved in studying living organisms…
Q: What are the types of wax bath?
A: There are many types of therapeutic baths that are prescribed by the physician according to the…
Q: Why would hydrogen peroxide be an effective disinfectant?
A: We have to answer why hydrogen peroxide can be an effective disinfectant.
Q: What is the purpose for testing a specimen for hydrogen sulfide production?
A: Hydrogen sulfide is produced when a bacterial strain reduces the sulfur compounds. The specimens…
Q: What would happen if you had skipped the heat fixing step and continued with the staining procedure?
A: There are various staining methods are used for staining the bacteria, namely simple staining,…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: What force was responsible for the movement of substances through the filter paper?
A: Filter paper is made up of cellulose and thus functions as permeable membrane and movement of…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Why is it necessary to use immersion oil when the 100X objective is used'
A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…
Q: How is fluoroscopy different from radiography?
A: Radiography and fluoroscopy are imaging techniques used in the medical studies. The Radiography or X…
Q: What happened when potato strip placed in salt water?
A: 1) A solution mixture that exhibits a higher concentration of salt than the surrounding medium. Then…
Q: What is the significance of reporting the color and consistency of a stool specimen? Explain…
A: One of the most crucial aspects of the treatment process is the diagnosis. They’ve ordered when…
Q: types of condensers are
A: The function of a Condenser in the microscope is to collect the light from light source and…
Q: What are the limitations in the use of potometer?
A: A potometer is also known as transpirometer. As the name suggests, the transpirometer is used to…
Q: What are the advantages of solid media over liquid media?
A: Solid media-It is a growth medium which forms solid support for the growth of colonies of…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: Of what practical importance are air borne microorganisms to the laboratory workers? What…
A:
Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…
A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: What are the three (3) possibilities of humidification process based on water temperature being…
A: Humidification is the process of adding moisture to the air. This can be done by water or steam to…
Q: Which is better to use in washing clothes, soap or synthetic detergent? Explain
A: Detergents are the surfactants or the mixture of surfactants. Surfactants are the compounds that…
Q: What steps are required to verify both contact with the sterilant and sterilization of an instrument…
A: Sterilization is the process that eliminates, removes, kills or deactivates the microorganism such…
Q: What products are sterilized using high energy ionizing radiation?
A: Sterilization is the process of killing microorganisms.
Q: What is the hazard of the spattering tendency in heating
A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…
Q: Write down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
A: It is a differential staining technique which was first developed by Ziehl and later on modified by…
Q: why is it necessary to use oil immersion on oil objective ?
A: The oil immersion is the technique of light microscopy, which is used to increase the resolving…
Q: How to reduce or eliminate aerial contamination inside the laboratory?
A: Indoor air quality (IAQ) : To main the indoor air quality becomes an important aspect especially in…
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: What is the purpose of setting the spectrophotometer to zero absorbance using a blank solution?
A: Spectroscopy is a procedure utilized for the evaluation, portrayal, and basic investigation of a…
Q: What are the different types of Pasteurization?
A: Pasteurization is the process of heat processing the liquid (mainly milk) or food so that the…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
What are the immersion media that need to be used?
Step by step
Solved in 2 steps
- How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.Explain briefly why the 100X objective lens of the light microscope is immersed in oil.
- a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?if 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved?
- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.Compare the two (or more) quantum dots emission at850 pbs/cds.. lnGaAs materials - which would be preferable for labeling certain molecules/organelles in biological samples for fluorescence microscopy? What were your considerations for this choice?You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective C
- If 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved? Explain your answer.when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across the DFV if they were laid end to end and 20 could fit if stacked side by side. What is the length and width of this organism in micra?You used the stage micrometers to measure the FOV for your microscope at 400x total magnification at 225 µm Use the formula Mag1 × FOV1 = Mag2 × FOV2 to calculate the FOV for your microscope at 1000x total magnification___________ 40x total magnification____________ Convert the 40x FOV to mm: _____________