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What could be done to further isolate organisms if multiple types of colonies were found on the plate streaked for isolation?
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- How do you recognize individual colonies? Describe color, shape, size. Explain how the streak plate method enabled you to identify a single species from a mixed culture?If you had a liquid culture containing three different species ofbacteria, how could the use of a Petri dish confirm this fact?How the use of a Petri dish could generate pure cultures of eachbody?In zone electrophoresis, why is it possible to isolate the components of a sample and recover them for identification? a) what influences the direction of migration of the components of a sample in zone electrophoresis?
- In what way could the extreme sensitivity of the PCR method be aproblem when working with clinical specimens?How could you determine if the white stringy material was DNA? A. By using spectrophotometer B. By adding ice cold ethanol C. By high speed centrifugation and observation of the pellet D. By deproteinization E. By looking at the unstained strings under the microscopeWhat staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well
- What would happen if the enrichment method in the isolation of bacteriophage was omitted?the chloroform was not added to the enrichment?the 0.1 ml lysate - E.coli mix was plated directly on top of the bottom agar?What are some of the problems that can arise when unstainedpreparations are enumerated in microscopic counts?If no amplified product was visualized by UV light on the gel, does it mean that there is no water-borne microbial pathogen in the unknown sample?