Why does fixation of the positive dye help bacteria stick to the slides?

Q: What is the purpose of the alcohol (OH) in the gram staining process?

A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: What is the correct shape of the unknown bacterium growing on your MacConkey agar?

A: Macconkey Agar This agar includes bile salts, which are found in the gut. Bile salts help in…

Q: Why is the Gram stain considered a differential stain?

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?

A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…

Q: why do some microbiologist use soil for DNA extraction

A: The DNA is exceedingly rare in the soil compared with that from plants, animals, fungi and microbes…

Q: Do we have to do an antibiotic sensitivity test to ensure the result? and what is the type and name…

A: Antibiotic Sensitivity Test:  Antibiotic sensitivity testing, often known as antibiotic…

Q: Why is it important for a microbiology laboratory technician to use aseptic technique?

A: The majority of the microbes onto which scientists work in laboratories are known pathogens.

Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?

A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…

A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…

Q: Which bacterial morphology is demonstrated by this simple stain? choose as many as are correct

A: For all types of bacterial cells, a simple staining method may be used to give contrast to the…

Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…

A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…

Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…

A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…

Q: Why do serial dilution? Does serial dilution make it easier to count bacteria on a spread/streak…

A: Dilution is the process of making a solution weaker or less concentrated.

Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…

Q: Why did the soap have to “cure” in the molds?

A: Fats and oils are triglycerides having a glycerol backbone with all the hydroxyl groups esterified…

Q: Why is it important to follow sterile technique?

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Why can't you see the bacteria when you first swab a plate

A: Bacteria are one-celled organisms that make up a vast group. They can dwell in a variety of…

Q: what is gram staining

A: Bacteria is a vast group of microorganisms that consists of various shapes, sizes, structure and…

Q: Is it likely that a pure culture could be isolated from the human body? Why or why not?

A: In microbiology the pure culture is a laboratory culture that contains a single species of organism.…

Q: How would the slide appear if the bacteria were stained with safranin, a basic red dye?

A: The appearance of bacteria, when stained with safranin, is to be determined.

Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…

A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?

A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…

Q: Why must young cultures be used when doing a Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: Based on the photo, describe the bacteria observed from the different staining technique. -…

A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…

Q: What was the purpose of leaving the cover on one set of agar plates? How did the growth on these…

A: The only microorganisms that can grow anywhere are bacteria. They can grow on specially prepared…

Q: Why is it called as differential stain

A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: Which bacterial species exhibited true motility on the slides?

A: Bacteria are single-celled prokaryotes ubiquitous in nature. As such, they can be found in different…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: Why do we need to stain microorganisms?

A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…

Q: how to find out the test is differential or selective in the microbiology lab test?

A: Many kinds of medias are used in microbial study. Two types of media having similar inferred names…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: What is the advantage of gram staining over simple staining

A:

Q: What is the purpose of iodine in the gram staining process? Explain.

A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…

Q: Why can't gram staining be used for mycobacterium?

A: Gram staining is one of the staining techniques. This is usually used for differentiating between…

Q: why is gram staining important

A: Gram staining is a laboratory technique which is used to differentiate between different types of…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: Why does  nigrosin not stain the bacteria?

A: Nigrosin is an acidic stain, used in the negative staining technique. Staining is a part of…

Q: Are there any chemical differences between the cell walls of gram-positive and negative bacteria…

A: Bacteria are the prokaryotes which fall under Kingdom Monera. They can be  found everywhere. They…

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…

Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…

A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…

Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…

A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…

Q: What staining method would you use to verify that the isolate is not producing survival structures…

A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…

Q: What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?

A: Streaking : Streaking for isolation on an agar plate involves the successive dilution of organisms…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."