Q: Define electrophoresis
A: Gel electrophoresis is a molecular biology technique used in laboratories.
Q: identiry at least four difere gel electrophoresis.
A: Four factors that will impact the how far molecules will move during gel electrophoresis 1. Sample…
Q: Why is dilution a necessary part of pure culture preparation?
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: Explain the process behind coulometry.
A: Coulometry is a method which is based on the measurement of the quantity of electricity which is…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a significant method for estimating restricting partiality among ligands…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: Create a flow chart on how gel electrophoresis is being conducted
A: Gel electrophoresis is a molecular technique that helps us to separate a mixture of DNA sample based…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: Transillumination is used clinically for the detection of:
A: Transillumination is an examination that is done with the help of a strong light shining on a…
Q: Why is the culture medium referred to as a highly enriched laboratory soil?
A: Culture medium is referred to as highly enriched laboratory soli because it provides all the…
Q: Discuss about the reagents used for agarose gel electrophoresis
A: Gel electrophoresis is used to separate nucleic acid.
Q: Define gel electrophoresis?
A: DNA is vital for all living beings. It is important for inheritance, coding for proteins, and the…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: Identify the following on the gel electrophoresis 2-leg ladder 3 Kb 1022 bp 1022 bp 880 bp 765 hp…
A: Biotechnology is the use of our understanding of biological processes to develop useful applications…
Q: Why is it important to use a sterilized loop between streaks when preparinga streakplate?
A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…
Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a…
A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…
Q: What are two types of compounds that make up the resin for column chromatography?
A: Column chromatography is a technique used for the separation or purification of biomolecules such as…
Q: Draw and explain the process of electrophoresis utilized in decalcification.
A: Decalcification :- As name suggests, it is removal of calcium salts,minerals and calcium tissues…
Q: Describe the agar-overlay technique
A: Biological techniques are methods or processes used to study living things. Methods, approaches,…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: Explain the function of stacking gel, separating gel, and Coomassie blue stain in the…
A: Answer : Stacking Gel : The purpose of the stacking gel packing is to combine all the samples of the…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: Why differential centrifugation can cause contamination in pellet?
A: Differential centrifugation is a typical process used in microbiology and cytology to separate…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: What is meant by the term “enrichment culture”?
A: The culture medium or growth medium is the specific medium, which contains all essential nutrients,…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…
A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…
Q: List four reasons for the development of pulsed field gel electrophoresis
A: Electrophoresis is a general term that describes the migration and separation of charged particles…
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: How could you utilize fluorescence quenching in the different analytical application? Please answer…
A: Fluorescence quenching is a method that diminishes the fluorescence force of an sample. A number of…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: Explain the conclussion about gel electrophoresis using 3 different food dyes (yellow, blue and…
A: The method used to separate mixtures of DNA, RNA, or proteins according to their size is known as…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…
A: Polychromatic solution of Methyl Blue and Eosin Y stains (modified Mann's solution) is used for…
Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…
A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…
A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…
Q: ative gel electrophoresis
A: Separation techniques are critical for identifying and isolating biomolecules for further biological…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: Explain the methods and materials in gel electrophoresis using 3 different food dyes (yellow, blue…
A: Gel electrophoresis is an analytical technique used in the lab to separate mixtures of DNA, RNA…
Q: What are the different manufacturing methods of Suppositories dosage form? Explain heat molding…
A: Suppositories are the solid medication that delivers drug in rectum ,vagina and or urethera. Most…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: In RPR test, Why is it necessary to rotate the slide/card?
A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.
Name the substance used as a medium in gel electrophoresis.
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