What is the significance of temperature control in the polymerase chain reaction?
Q: How Polymerase Chain Reaction (PCR)Amplifies Defined Regions of a Genome?
A: Polymerase chain reaction or PCR technique is used to amplify or make multiple copies of a specific…
Q: Compare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.
A: Replication is a process, which involves the synthesis of DNA from the template DNA, with the help…
Q: Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain…
A: Polymerase (PCR) chain reaction is a process, during which the specific DNA segment is copied in a…
Q: What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that…
A: Deoxyribonucleic acid or DNA is a nucleic acid that composed of two polynucleotide chain that is…
Q: Why Sanger Sequencing Dependson DNA Polymerase?
A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…
Q: Which of the following polymerase Il play a key role in? Please make sure to select ALL correct…
A: RNA polymerase II is an enzyme found in eukaryotic cells . Precursors of mRNA are formed by…
Q: What molecules or structures are present in cell that might interfere with DNA extraction?
A: DNA extraction is a method used to purify DNA from a sample. Here seperation of DNA occur from…
Q: Discuss how the polymerase chain reaction works.
A: Polymerase chain reaction can be a method that will be used to rapidly make millions to billions of…
Q: What is B-form DNA?
A: DNA is the main constituent of the chromosome. It contains all information about protein that forms…
Q: What is clone in terms of cells?
A: Introduction All cells with some exceptions undergo cell division in order to multiply. During cell…
Q: How does proofreading take place in the process of DNA replication?
A: DNA replication is the process of producing two identical replices of DNA from one original DNA…
Q: Describe and give the results of an experiment that shows that polymerase I is located primarily in…
A: The eukaryotes have three RNA Polymerases These RNA Polymerase I , II and III, have different…
Q: The polymerase chain reaction can only be used to amplify genes that have been cloned and sequenced.…
A: The polymerase chain reaction was originally developed by the American biochemist Kary Mullis. The…
Q: How did Kornberg assess the fidelity of DNA polymerase I in copying a DNA template?
A: Deoxyribonucleic acid (DNA) is a particle made out of two polynucleotide chains that loop around one…
Q: How would a researcher determine if a new species of bacteria contains the gene for producing DNA…
A: The prolonged exposure of DNA to UV radiation will result in mutation, where the thymine residues…
Q: What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction…
A: Thermus aquaticus is a heat tolerant bacterium .DNA polymerase used from Thermus aquaticus is heat…
Q: Which enzyme is used in Sanger sequencing reactions? O A. DNA polymerase O B. S1 endonuclease O C.…
A: Introduction :- Sanger DNA sequencing is commonly employed in research to target smaller genomic…
Q: What is the process of transformation in bacteria?
A: Transformation of bacteria and certain other cells is a versatile tool in genetic engineering. The…
Q: Why is temperature control so important in the polymerase chain reaction?
A: The polymerase chain reaction (PCR) is used to make millions of copies of a small sample of a…
Q: What are the essential requirements for the polymerase chain reaction
A: Polymerase chain reaction (PCR) is the laboratory technique used to amplify specific piece of sample…
Q: What are bypass polymerases? How do they differ fromthe replicative polymerases? How do their…
A: Polymerases are enzymatic proteins that has a specific role in maintaining the overall molecular…
Q: What is Taq polymerase and why is it important?
A: DNA Polymerase is a very important enzyme in the process of DNA Replication. It is a process in…
Q: what are some Limitations/sources of error with suitable rationalization that may occur during the…
A: PCR is in vitro method for amplifying defined target DNA sequences present within the source of DNA.…
Q: What are the functions of primer and Taq polymerase?
A: Polymerase chain reaction used to make thousands of copies of DNA from very small amount of the DNA…
Q: Briefly discuss Polymerase chain reaction and Taq polymerase
A: Polymerase chain reaction used to make thousands of copies of DNA from very small amount of the DNA…
Q: What is the meaning of conjugation in biology?
A: Biology is the division of science that deals with life and living organisms, like their structure,…
Q: In DNA amplification, using the Taq polymerase, what is the maximum number of amplification cycles?…
A: Introduction In the field of molecular biology, however, cloning is viewed at a genetic molecular…
Q: Do DNA-polymerase enzymes also function as exonucleases?
A: DNA polymerase enzyme is primarily responsible for the synthesis of DNA by the attachment of various…
Q: How is the genome replicated prior to binary fission in a bacterial cell?
A: DNA replication is a process by which duplication of a molecule of DNA takes place. Duplication of…
Q: How Are RNA Genomes Replicated
A: Most of the genomes of viruses composed of RNA and replicated through use of RNA or as a template…
Q: what is the Compares and contrasts transformation, transduction, and conjugation
A: In transformation, a bacterium takes up a piece of DNA floating in its environment. In transduction,…
Q: What processes are involved in bacteriophage assembly?
A: A bacteriophage or phage is a type of virus that infects bacteria, as indicated by the name which…
Q: What will happen if DNA copying mechanisms were to be less accurate?
A: BASIC INFORMATION DNA Replication It is known as copying of the DNA. It takes place in the…
Q: Describe the effects of the temperature change during polymerase chain reaction
A: Polymerase chain reaction or PCR is an in vitro technique in which replicas of DNA are synthesized .…
Q: Has all of the DNA been replicated during Sphase?
A: DNA or deoxyribonucleic acid is a biomolecule consisting of nucleic acids as monomer units. Nucleic…
Q: Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?
A: Polymerase chain reaction or PCR is a technique applied on DNA. DNA is a polymer of multiple…
Q: What is the transformation factor?
A: The process in which the genetic material formed of the naked DNA (deoxyribonucleic acid), which is…
Q: What is the significance of using thermostable DNA polymerase for Polymerase Chain Reaction?
A: Polymerase chain reaction (PCR) is a technique for producing multiple copies of DNA in in-vitro…
Q: What is natural transformation in biology?
A: In biology transformation is the process of genetic alteration of a cell. In this process an…
Q: Which E. coli enzyme is the main replicative polymerase in bacteria that synthesizes the genome?
A: Replication is the process by which new DNA strands are duplicated from the existing DNA strands. It…
What is the significance of temperature control in the polymerase chain reaction?
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Solved in 2 steps
- how do the high temperature affect polymerase to make a mistake as our body temperature will not be too high?Briefly describe the polymerase chain reaction. What is the difference between a step and a cycle?Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain reaction?
- The synthetic unit of the polymerase chain reaction is the replica. True or false?What difficulties arise in the polymerase chain reaction if there is contamination of the DNA that is to be copied?Why is the use of temperature-stable DNA polymerase animportant factor in the polymerase chain reaction?
- What is the significance of using thermostable DNA polymerase for Polymerase Chain Reaction?Assume that a cycle of the polymerase chain reaction takes 20 minutes. How many copies of a single DNA fragment will there be after the reaction has been run for six hours?Can you explain what is nucleotide selectivity in polymerase?