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- what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the purpose of each variant in clinical diagnosis?How does plating efficiency affect the number of plaque-forming units? How is plating efficiency calculated?In ELISA, what is the importance of washing? When does washing is performed?
- What is the importance of washing the ELISA plate?Can you show the step by step process of Voges Proskauer Test using a schematic diagram?What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?
- Is there any way to stabilize color after adding stop solution in ELISA in Which HRP and TMB used as enzyme and chromophore?Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of pure cultures in thelaboratory?