What is meant by the word sterile? Why is aseptic technique
necessary for successful maintenance of pure cultures in the
laboratory?
Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on the surface of living organisms. The word sterile originally means that the context in question is incapable of 'producing' anything. Thus with regard to microbiology, the word sterile means aseptic, free of any microorganisms whatsoever.
Microbes are present everywhere, and survive on their metabolic bare minimum requirements. If provided with conducive conditions, they can thrive and multiply.
A pure culture in biology is one in which only a single species of bacterium (typically, but also pertains to fungal cultures) is growing.
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