What information do we get if we calculate the ratio of the Absorbance value at 260 nm to the Absorbance value at 280 nm?

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter16: Adult And Pediatric Dosages Based On Body Surface Area
Section: Chapter Questions
Problem 4.1P
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2247
22.00
22.00
20.00
18.00
16.00-
14.00
Sample type
HINA 40
Sampie D
12.00-
10.00
9 8.00-
6.00-
4.00
2.00
Samplee
20 Abs 224
4-260 10 mm path (23.055
A-280 10 mm path (11.20
200/280 2.00
200/230 1
0.00
-2.55
220 230 240 250 260 270 282 290 300 310 320 330 340 350
nglu s222
Wavelenath nm
We have extracted nucleic acids from tissue. A spectrophotometer was used to
analyze the samples.
What information do we get if we calculate the ratio of the Absorbance value at
260 nm to the Absorbance value at 280 nm?
10 mm Absorbance
Transcribed Image Text:2247 22.00 22.00 20.00 18.00 16.00- 14.00 Sample type HINA 40 Sampie D 12.00- 10.00 9 8.00- 6.00- 4.00 2.00 Samplee 20 Abs 224 4-260 10 mm path (23.055 A-280 10 mm path (11.20 200/280 2.00 200/230 1 0.00 -2.55 220 230 240 250 260 270 282 290 300 310 320 330 340 350 nglu s222 Wavelenath nm We have extracted nucleic acids from tissue. A spectrophotometer was used to analyze the samples. What information do we get if we calculate the ratio of the Absorbance value at 260 nm to the Absorbance value at 280 nm? 10 mm Absorbance
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