When doing the glucose in drinks experiment I measured an absorbance reading of 0.002 for orange juice and 0.045 for iced tea which is essentially nothing, why would this be the case
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When doing the glucose in drinks experiment I measured an absorbance reading of 0.002 for orange juice and 0.045 for iced tea which is essentially nothing, why would this be the case
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- Would a sample solution that has a high absorbance reading, have a low or a high concentrationWhen measuring the absorbance of methylene blue solution containing corn starch, how would corn starch affect the absorbance of the solution if it is not completely filtered out? Explain. (Don't just say "it affects" but explain it increases/decreases the absorbance. )Normal room temperature is about 74 degree. What is the equivalent temperature on the Celsius scale?
- If you had measured the absorbances of your unknown in test tubes that were half the diameter of the ones that you used to make the standard curve, how would the mg of protein in the samples be affected? (Higher, Lower, No Change)When bound to proteins, the Bradford reagent turns blue. You measured the absorbance of your samples at 595 nm, which corresponds to yellow light. Why did you measure the absorbance using yellow light instead of blue light?Determine the amount of glucose in the unknown sample by plotting a standard curve of Absorbance at 620 nm on Y-axis and μg of glucose on the x-axis.
- You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equationBuffer systems can absorb hydrogen ions from the blood to increase pH into homeostatic range (pH: 7.35 to 7.45)During what condition would buffer systems do this?Use the equation of your line to calculate an accurate concentration of solute if the absorbance is 0.15 AU. Show your calculations.
- Compute for the sodium concentration of the patient. Use the same formula in the computation of the concentration using absorbance and concentration of the standard. Given: Standard sample Concentration : 150mmol/L Absorbance: 0.112 Patient's sample Absorbance : 0.226 Concentration : ??Name the two component that contribute to the electrochemical gradient (hint is in the name!) and briefly describe what they are.Why different molecules have different absorbance wavelengths (nm)? Please answer shorty at your own words. Answer should be to the point.