help please with calculations Below is a BSA standard curve and the duplicate values of absorbance for 3 samples.Calculate the concentration of BSA in each sample and say if the standard curve is adequatefor each of these samples? Why or why not? If not, what would you do to fix this? Show yourcalculations.Absorbance10.90.80.70.60.50.40.3y = 0.9825x - 0.1079R² = 0.9910.20.10.20.40.60.811.2BSA concentration % (m/v)Curve equation: y=0.9825x-0.1079R?=0.991AbsorbanceSampleReading 1Reading 2Sample A0.5780.536Sample BSample C0.9661.0420.1840.203Absorbance

Lambert Beer law states that Absorbance of a solution is directly proportional to its concentration…

Answered: Below is a BSA standard curve and the…

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

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A calibration curve for the absorbance of an unknown is shown below. What would be the concentration of a sample with absorbance of 0.45. Give the answer with the correct unit.
In this question, you need to discuss the results you obtained from the BSA standards to generate your standard curve. Are you confident in the absorbance values you measured from the BSA standards? Was there variability between your duplicates? Comment on the shape of your standard curve and note the highest absorbance you would consider for interpolating the concentration of an unknown sample.
You record the absorbance of your unknown BSA sample at 0.276. What is the concentration in ug/mL of the unknown assuming you have a standard curve with the line of best fit with y=0.0001x+0.0038 as its equation
If you had measured the absorbances of your unknown in test tubes that were half the diameter of the ones that you used to make the standard curve, how would the mg of protein in the samples be affected? (Higher, Lower, No Change)
What is the relationship of concentration and absorbance based on the table and what are the changes in the absorbance value mean?
i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).
Given the equation of the line, y=150x-8.1221 (cells per mL is in 10^8), what is the CFU per mL of the original suspension if the thousand-fold dilution of that suspension has an absorbance of 0.3 at 600nm? * A. 3.7 x 10^12 B. 3.7 x 10^9 C. 3.7 x 10^1 D. None of these is correct
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.284 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
How many mL of methadone is needed for a patient who was prescribed 0.015 g of methadone? The vial reads 6 milligrams of methadone per 1 mL of solution.
A 46.60-gram pycnometer was filled with 100.50 grams of liquid. When filled with insoluble powder, the pycnometer weighs 84.60 grams. When the insoluble powder and liquid were combined, the pycnometer weighed 172.8 grams. What is the relative density of the insoluble powder?
An analysis of city drinking water for total hardness was done by two students in the laboratory and produced the following results (in ppm CaCO3): Student A: 228.3, 226.4, 226.9, 227.1, and 228.6. Student B: 229.5, 226.1, 230.7, 223.8, and 227.5 1. Calculate the following for each data set: (proper significant figures, neglect units) a) Mean for student A b) Mean for student B c) Relative standard deviation for student A d) Relative standard deviation for student B e) Confidence Level for Student A at 95% (write as a range for example: 200-300) f) Confidence Level for Student B at 95% (write as a range for example: 200-300) g) Which of the student have a more precise result? (write STUDENT A or STUDENT B)
If 400 mL of a 20% w/v solution were diluted to 2 L, what would be the finalpercentage strength?

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Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the concentration of BSA in each sample and say if the standard curve is adequate for each of these samples? Why or why not? If not, what would you do to fix this? Show your