What is a recombinant DNA? Select one: a. During meiosis, sister chromatids cross over to produce daughter cells with recombinant DNA. b. Any genome that is non-parental is called a recombinant DNA. c. New DNA sequences are inserted into a genome to produce recombinant DNA. d. Bacterial plasmid with sticky ends are referred to as the recombinant DNA.

What is a recombinant DNA? Select one: a. During meiosis, sister chromatids cross over to produce daughter cells with recombinant DNA. b. Any genome that is non-parental is called a recombinant DNA. c. New DNA sequences are inserted into a genome to produce recombinant DNA. d. Bacterial plasmid with sticky ends are referred to as the recombinant DNA.

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 4RQ: What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel...

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Why must a genetically engineered plasmid contain a genetic marker? a.) to prevent the construction of an artificial chromosome b.) to separate cells that contain recombinant DNA from those that do not c.) to produce multiple copies of the recombined plasmid after heat treatment d.) to break apart the circular plasmid and introduce another DNA fragment
What is the function of resolvase in recombination? a. It unwinds double-stranded DNA. b. It allows a single DNA strand to invade a DNA helix. c. It displaces one of the original DNA strands during branch migration. d. It cleaves the Holliday structure.
From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase B) they are cut out of plasmids using restriction endonucleases C) DNA primase is added to the sequencing reaction and synthesizes the primers D) biotechnology companies synthesize them using organic chemistry
What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' direction
Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.
What is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbones
Refer to the DNA models below. a. In what ways are they similar?b. In what ways are they different?
What is DNA polymerase? A. An enzyme that corrects mutations that arise during the replication of DNA B. An enzyme that seals any gaps that remain between bases of replicating strands of DNA C. Short, single strand of DNA that base-pairs with a specific DNA sequence D. An enzyme that carries out DNA replication
in the human genome project, scientists have identified the sequence of the base pairs of the human chromosome, and are working to determine what?
If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organism
The polymerase chain reaction (PCR) is important because it allows us to Group of answer choices a. insert eukaryotic genes into prokaryotic plasmids b. incorporate genes into viruses c. make DNA from RNA transcripts d. make many copies of a target segment of DNA
Diagram and describe the methods of PCR, DNA sequencing, Restriction Enzymology, & Hybridization techniques. - How were these methods mostly derived from knowledge of DNA structure and function?