About transcriptomics and genomics, answer:4.Can we replicate RNA? How can we use PCR and Sanger sequencing to study theranscriptome?a.b.How will the genome of two different cells of the same animal compare?How will the transcriptome oftwo different cells of the animal compare?c.

Question
Asked Nov 5, 2019
About transcriptomics and genomics, answer:
4.
Can we replicate RNA? How can we use PCR and Sanger sequencing to study the
ranscriptome?
a.
b.
How will the genome of two different cells of the same animal compare?
How will the transcriptome oftwo different cells of the animal compare?
c.
help_outline

Image Transcriptionclose

About transcriptomics and genomics, answer: 4. Can we replicate RNA? How can we use PCR and Sanger sequencing to study the ranscriptome? a. b. How will the genome of two different cells of the same animal compare? How will the transcriptome oftwo different cells of the animal compare? c.

fullscreen
check_circleExpert Solution
Step 1

(a)

RNA viruses can replicate their RNA. RNA can store and replicate genetic information. RNA dependent RNA replication is a special process, that is reserved for RNA viruses exclusively, but not for cellular RNAs.

RT-qPCR (real time PCR) is widely used in the study of transcriptome. We can use qPCR for the analysis of gene expression in the transcriptome study. Sanger sequencing is an old method of DNA and RNA sequencing. In Sanger sequencing method in the first step we have to convert the population of RNA to be sequenced into the fragments of cDNA that means cDNA library, after that we do RNA sequencing that will used in the transcriptome study.

 

Step 2

(b)

The different cells of an organism contain the same DNA. The cell types of organism become different because they synthesize different RNA sets and protein molecules, but they do thi...

Want to see the full answer?

See Solution

Check out a sample Q&A here.

Want to see this answer and more?

Solutions are written by subject experts who are available 24/7. Questions are typically answered within 1 hour*

See Solution
*Response times may vary by subject and question
Tagged in

Science

Biology

Molecular Biology

Related Biology Q&A

Find answers to questions asked by student like you

Show more Q&A add
question_answer

Q: If the coding region of a gene (the exons) contains 2,100 base pairs of DNA, would a missense mutati...

A: A missense mutation causes change in a single nucleotide. The resultant codon codes for an amino aci...

question_answer

Q: In horses, black coat (B) is dominant to brown coat (b), and being a trotter (T) is dominant to bein...

A: In horses black coat (B) is dominant trait to brown coat (b), and trotter (T) is dominant trait to p...

question_answer

Q: Which of the following is the most comprehensive definition of metabolism in living organisms? Meta...

A: Metabolism is set of processes that is necessary for life.Metabolism, the sum of the chemical reacti...

question_answer

Q: Why and under what conditions will air enter into and out of the lungs?

A: Lungs lie on each side of your breastbone and fill the inside of your chest cavity. It is one of the...

question_answer

Q: Unlike skeletal muscle smooth muscle Mayer spontaneously contract when it is. What feature of smooth...

A: Smooth muscle is a non-striated muscle, divided into two groups; single unit and multiunit smooth mu...

question_answer

Q: The Miller-Urey experiment used a closed system containing simple gases like ammonia, methane and wa...

A: The Miller-Urey experiment was performed by Stanley Miller and Harold Urey. This experiment simulate...

question_answer

Q: In our class of 130 students, 64 (49%) are unable to taste PTC. What is the percentage of individua...

A: PTC tasting ability is a simple genetic trait that is dominant. It is governed by a pair of alleles,...

question_answer

Q: Explain what is happening when the appendix or tonsils get swollen.

A: A pair of soft tissue masses in the throat, one on each side of the tongue root is termed as tonsils...

question_answer

Q: The bioavailability of which two vitamins is significantly higher in supplemental form as compared t...

A: Vitamins are organic molecules that are required in very small quantities but play a vital role in p...