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What is cooperativity, and why does it not require that
“cooperating” sites affect each other directly?
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- Is the dependent variable the reaction time? And what is the controlled variables in this experiment and are there any controls that are used in this experiment?Non-specific interactions present a real challenge to co-IP experiments because non-physiological binding to the target complex is likely to occur. Group of answer choices True FalseFor a protein that binds multiple ligands with negative cooperativity, which of the following is true? Each ligand bound increases the dissociation constant for subsequent ligands. Ligands cannot bind to the protein. The dissociation constant is not affected by binding of other ligands. Each ligand bound decreases the dissociation constant for subsequent ligands.