what is spectrophotometry? how is it related to Beer's Law?

Q: Why is a suppressor used in ion chromatography?

A: Ion chromatography- It is used for water chemistry analysis. Ion chromatography helps in measuring…

Q: Describe what is chromatography?

A: Introduction: There are various methods to separate the mixtures like distillation, physical and…

Q: Which technique is based on the stronger attractionsome components have for the stationary…

A: In chemistry, when a reaction is performed between the reactants, that just does not involve mixing…

Q: Is the separation of the mixture shown here a physical orchemical process?

A: SOLUTION: Step 1: The separation of the mixture shown above (i.e. by column chromatography) is a…

Q: chromatography

A: chromatography is a process in which the mixture is separated.  In this process we dissolve the…

Q: Q.No.3 What is chromatography?. Why chromatography is considered as an important tool for analysis…

A: A question about Chromatography, which is to be accomplished.

Q: Name three common uses of High Performance Liquid Chromatography (HPLC) in the laboratory.

A:

Q: Red, blue, and yellow spots were found in the chromatogram. Red spot traveled twice the distance…

A:

Q: Although TLC is qualitative and tells you what is present in a given sample, it can also be used to…

A: Thin layer chromatography (TLC) is a part of types of chromatography techniques that is used in the…

Q: 1. State the mathematical equation known as Beer's law. Define each symbol.

A:

Q: 6. What causes the components in a mixture to separate in a chromatography experiment?

A: Chromatography is the special type of experimental procedure that can be used for separation of…

Q: How does paper chromatography allow you to separate substances?

A: Paper chromatography allows you to separate substances has to be determined below.

Q: 1. Describe the operation of spectrophotometer.

A:

Q: What is wrong with this picture? TLC PLATE

A: Given  a TLC paper

Q: 7. What experimental factors are assumed to be constant in this experiment?

A: (7) Consider the ideal gas equation is as follows;      PV = nRT…

Q: _is a technique which is used to separate a single chemical 3. compound from a mixture dissolved in…

A: Chromatography can be defined as the technique used for separating mixtures with the help of a…

Q: In analytical chemistry, when and why do we do representative sampling? How can you apply this…

A: A question based on analytical chemistry, which is to be accomplished.

Q: In three sentences, describe the principle of chromatography.

A:

Q: What precautions should you take in handling a cuvet and placing it in the spectrophotometer?

A: Interpretation: The precautions taken in handling a cuvet and placing it in the spectrophotometer…

Q: A student performs a TLC experiment and at the end of the experiment, sees no spots other than the…

A: TLC stands for thin layer chromatography. This is a very useful technique to separate and identify…

Q: a] An analyst would like to analyze the concentration of Mg2+ in a solution. He needs a calibration…

A: A question based on absorption that is to be accomplished.

Q: stationary phase and mobile phase

A:

Q: Julie has been asked to measure the concentration of a sample solution using a spectrophotometer.…

A: On dilution, the no of moles does not change. As no of moles = Molarity × Volume Thus, (Molarity ×…

Q: Principles of Chromatography

A: Definition :

Q: Which one is the most common method used for the purification of liquid mixtures? Filtration…

A: Different methods are used purification of compounds.

Q: Explain why the concentration of zinc cannot be determined directly using a spectrophotometer.

A: The atomic no of zimnc is 30. It contain many free electrons

Q: When using cuvettes to measure the absorbance of a solution, why is it necessary to only hold the…

A: Generally during the measurement of absorbance of a solution ,we hold the cuvettes near the top ,due…

Q: What are the main components of a spectrophotometer? Choose all that applies. radiation source…

A:

Q: A gel filtration column has a radius (r) of 0.80 cm and a length (l) of 20.0 cm. Calculate the total…

A:

Q: What compounds present in a sample that can cause error in the analysis and alter desired results?…

A: Interpretation - We have to tell that due to which  compounds present in a sample that can cause…

Q: what ccan be a cause of not getting a baseline separation when it comes to chromatography? if it is…

A: chromatography It is the separation technique that involves the principle of affinity of the…

Q: Chromatography is a separation technique. What is the physical or chemical basis for separation of…

A: The separation relies on the relative affinity of compounds towards both the phases. The compounds…

Q: 1. From spinach, the chromatographic analysis will yield two major substances: (a) B-carotene…

A: (i). Characteristics of beta-carotene: β-carotene is the molecule that gives carrots, sweet…

Q: How can TLC be used to determine if a sample is pure?

A: Thin Layer Chromatography (TLC) is a separation procedure that requires a very small sample. It is…

Q: 5. What would be the effect of the following errors in chromatographic work? a. The solvent level in…

A:

Q: The TLC and mp data contradict one another with regards to purity. True False

A:

Q: On what principle is chromatography based?

A: The mixture of the various compounds can be separated by several separation techniques. In one of…

Q: 50.00 45.00 40.00- 36.00 30.00 3 25.00 20.00 15.00 10.00 5.00 MM CM 0.00 0.00 120 6.00 10.00 1 14.00…

A: Area of sorbitol peak is calculated in the following step.

Q: what chromatography seperation how is tlc part of it

A:

Q: Station 2: Magnesium and Hydochloric Acid Observatons:

A: When we are giving it that fire magnesium strip would just go away and  leaving nothing behind. Also…

Q: What is affinity chromatography? What 'are the principles of affinity chromatography? which…

A: Answer -  Affinity chromatography - Affinity chromatography is a separation method based on a…

Q: Assume the data below was collected for two different sample solutions using the same experimental…

A:

Q: [Glucose), mg / mL | Absorbance 0.132 5.000 10.00 20.00 30.00 0.290 0.498 0.642 40.00 0.750 a. Using…

A: a) In the given table, the absorbance data of ferricyanide ion is given on varying the concentration…

Q: Give the general steps in the use of a spectrophotometer.

A: A question based on spectrophotometer, which is to be accomplished.

Q: A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. You subject it to…

A: We have given that   Absorbance (A) = 0.545  Mass of amine = 0.1 g  The molar absorptivity (ε) =…

Q: 4. Why is it so important that you don't erase mistakes on the chromatography раper?

A: Given : Chromatography paper To Find: Reason for not erasing mistakes on chromatography paper…

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