[Glucose), mg / mL | Absorbance 0.132 5.000 10.00 20.00 30.00 0.290 0.498 0.642 40.00 0.750 a. Using the method of least-squares, give the equation of the best straight line through the points. b. Calculate the concentration of an unknown solution that gave an absorbance of 0.440. c. Give the correlation coefficient R and tell whether it is a good fit line or not.
[Glucose), mg / mL | Absorbance 0.132 5.000 10.00 20.00 30.00 0.290 0.498 0.642 40.00 0.750 a. Using the method of least-squares, give the equation of the best straight line through the points. b. Calculate the concentration of an unknown solution that gave an absorbance of 0.440. c. Give the correlation coefficient R and tell whether it is a good fit line or not.
Chapter24: Introduction To Spectrochemical Methods
Section: Chapter Questions
Problem 24.16QAP
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The concentration of glucose in an aqueous solution can be determined by oxidation with ferricyanide ion, Fe(CN)6^3–. The absorbance of the ferricyanide ion measured at 420 nm increases as the concentration of glucose increases.
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