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What is the basic principle of GPC (gel permeation chromatography)?
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- What is the principle of the gel electrophoresis separation technique?In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?What types of macromolecules are usually separated on agarose electrophoresis gels?
- Of the two principal polymers used in column chromatography and electrophoresis, which one would be most immune to contamination by bacteria and other organisms?What is Xanthan ? What is the importance of using Xanthan in the emulsion experiment?What is the basic purpose of the paper chromatography?
- Why is the order of separation based on size opposite for gel filtration and gel electrophoresis, even though they often use the same compound to form the matrix?What are the benefits and disadvantages of IMAC for purifying proteins?In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?