What is the chemical basis of Molisch test?
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Q: 1. What is the chemical basis of Molisch test?
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- Identify which one among the 6 carbohydrates; Glucose, fructose, maltose, lactose, sucrose or starch is present in the sample, if the sample gave the following test results. Positive results in Molisch, Benedicts and Seliwanoff's tests. Negative results in lodine, Bials and Mucic acid tests. An instant positive result in Barfoed's test.https://www.youtube.com/watch?v=rKng5-ij6kQ Provide a schematic diagram for the Molish test methodologies in determining the presence of carbohydrates. Also, give the basic principle for the test. (not a graded question, the video is very brief) (please take time to answer)Reagents: 8 ml conc. Sulfuric acid, 1.5 ml alpha naphthol, 1 ml iodine in KI, 4 ml conc. HNO3, 4 ml Seliwanoff’s reagent, 3 ml Bial’s-orcinol reagent, SAMPLE: 5% of lactose, sucrose, glucose, galactose, fructose, maltose, starch and xylose ( 10 ml each) Materials: 12 test tubes 20 ml, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, 3 droppers, 250 ml beaker Procedure: 1. Molisch Test – (use all 8 samples) Place 1 ml of each sample in different test tubes. Add 1 drops of alpha napthol reagent in each tube. Mix thoroughly. Incline the tube and CAREFULLY allow 1 ml of conc. H2SO4 to flow down the side of the tube. Note the color formed at the zone of contact between 2 liquids. 2. Iodine Test – ( use all 8 samples) place 1 ml of the sample in a test tube. Add a drop of iodine. Observed color produced. Heat (direct heating) and note change in color. Cool and record again the result.…
- Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Which test can be used to differentiate fructose and glucose? Barfoed’s Test Bial’s Orcinol Test Molisch Test Iodine Test Benedict’s Test Seliwanoff’s TestMatch the results to the name of the test. (+) Glucose and (-) Sucrose (+) Brain sugar and (-) Levulose (+) Agar and (+) Starch (+) Brain sugar and (-) Milk sugar (+) Sucrose and (-) Glycogen (+) Xylose and (-) Dextrose (+) Starch and (-) Gelatin (+) Levulose and (-) Beet sugar - Barfoed's Test - Mucic acid Test - Bial's Test - Seliwanoff's Test - Nylander's Test Refer to the image below and select two correct statement.
- Which of the different carbohydrate tests would give a positive result for maltose? Describe the color change. Molisch test Fehling’s test Benedict’s test Tollen’s Test Barfoed’s test Seliwanoff’s test Phenylhydrazine or osazone testDESCRIBE THE RESULTS OF THE REDUCTION TEST FOR SUGARS USING THE FOLLOWING TEST PICRIC ACID TEST SUGAR RESULT 1. Glucose 2. Sucrose 3. Maltose 4. Fructose 5. Lactose 6. GalactoseTrue or False Galactose gives a positive result with Benedict’s test faster than lactose due to the acidity of the reagent used.
- Which test can be used to differentiate ribose and glucose? Benedict’s Test Iodine Test Barfoed’s Test Seliwanoff’s Test Molisch Test Bial’s Orcinol TestWhich of the following tests is used to distinguish glucose from fructose?* A. Molisch test B. Seliwanoff's test C. Bial's Orcinol D. Kowarsky test E. None of the given optionsThe table below summarizes the results for Ninhydrin test. Provide the correct remarks from the results if it's either Positive for Ninhydrin test or Negative for Ninhydrin test. and if it's Positive for α-amino acid or Negative for α-amino acid.