Why do we add powdered zinc to the nitrate reduction test?

Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…

A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…

Q: What is the result for the yellow tube of Methyl red test? What must the pH be in each tube? What is…

A: Methyl red test This test usually aims to differentiate the facultative anaerobes enteric bacteria.…

Q: Oven sterilization at 121˚C was performed on glassware for 15 minutes only. Effect:  Rationale:

A: Sterilization  It is defined as the process in which micro-organisms whether bacteria, fungi,…

Q: Why is dilution a necessary part of pure culture preparation?

A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…

Q: What is this test? What coated the bottom of the wells? What were we testing for? How did we know it…

A: The test is ELISA. It is an Enzyme linked Immunosorbant Assay, which is used in many applications.…

Q: Write the materials,  reagents, Principle and  procedure for the Periodic acid Schiff technique

A: Answer: STAINING = It is the method to colorize the smear or transparent tissue or cells in order to…

Q: What is the result of tube A in this Voges-proskauer test? What are the reagents of this test? What…

A: Voges-proskauer test is performed to differentiate between facultative anaerobic enteric bacteria.

Q: Why should the use of nichrome or iron containing loop/wire be avoided as an inoculating device when…

A: The oxidase is a test used in microbiology to identify bacteria that produce cytochrome c oxidase.…

Q: What are the advantages of the API 20E test compared to regular biochemical tube media

A: API 20E test helps in the determination of bacteria from lab-scale activated sludge treatment…

Q: Why is betadine a good DIY substitute for gram’s iodine or dissolved Potassium iodide which is…

A: Iodine, with the symbol I and the atomic number 53, is a chemical element. At typical conditions, it…

Q: Methyl Red test What color is the ph indicator in the dropper bottle?

A: Methyl Red test is a quantitative biochemical test, which is widely used to detect the ability of…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: What are the advantages and disadvantages of the filter method in the analysis of water samples?

A: Utilising the membrane filter method, test is gone through the layer utilising a filter funnel and…

Q: Why do the results for the confirmed test represent  faecal coliform in the analysis of water using…

A: MPN is Most probable number. It is commonly applied for testing the quality of water.

Q: Which is the better reagent for the clinical test between Fehling's solution and Benedict's…

A: The reducing sugars get oxidized by reducing some other component. This reducing property of these…

Q: Draw/illustrate the chemical reaction involved in Lugol’s test and explain the purpose of this…

A: Carbohydrates are polymer molecules that are composed of monosaccharide molecules. Monosaccharides…

Q: Why would hydrogen peroxide be an effective disinfectant?

A: We have to answer why hydrogen peroxide can be an effective disinfectant.

Q: yield

A: The Dische's test is a chemical test that is utilized to detect the presence of DNA in a solution.It…

Q: What is the purpose for testing a specimen for hydrogen sulfide production?

A: Hydrogen sulfide is produced when a bacterial strain reduces the sulfur compounds.  The specimens…

Q: When is sterilization by filtration preferred over sterilization by heat? Why? What makes heat an…

A: Answer :- 1)Filteration is the sterilization process wherein there is the physical removal of…

Q: What are high level disinfectants? What is an example of one? What is the method of action and the…

A: Disinfectants are chemicals that are used to kill the microorganisms present in the surroundings.…

Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…

A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…

Q: What advantages do solid media offer for the isolation of microorganisms?

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable…

A: Harada-Mori culture technique It is a method of incubating fecal material on a filter paper strip in…

Q: A carbohydrate sample reacts positively with all test reagents except for Lugol's. The sample could…

A: Carbohydrates are the chief biomolecules which act as energy source. Most of the body cells depend…

Q: Why is there a need to use acids and boil the sample in maceration?

A: Maceration is a common method of obtaining medicinal plants, it is an extraction process in which…

Q: What is the difference between a Hemin test and a Benzidine test?

A: Hemoglobin is a blood protein that is responsible for oxygen transport in the tissues. Hemoglobin…

Q: what is oil immersion and why is it used

A: Light microscope is a microscopic instrument which uses visible light and magnifying lens to to view…

Q: Why does the oxidase test must be performed from blood agar or another medium without a fermentable…

A: Biochemical tests are used to identify microbial diversity based on variations in the biochemical…

Q: Why is the incubation temperature criticalwhen using the Filter Method when testing water samples?

A: Incubation temperature is critical for bacterial growth if this temperature is too high or too low…

Q: What is the importance of the Nitrate test? What information can we gather from this test? Please…

A: Nitrate broth used in the nitrate test contains nutrients and potassium nitrate as a source of…

Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…

A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…

Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?

A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…

Q: what is sterilization or define sterilization?

A: Sterilization is defined as the process where all the living microorganisms, including bacterial…

Q: What is the most commonly used fixative in stool samples and why?

A: Fixative is a moist environment for transporting and preserving intestinal parasites in stool…

Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…

A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…

Q: Why are only few drops of CuSO4 solution added during the biuret test?

A: Biuret test also known as Piotrowski's test, is a chemical test used for detecting the presence of…

Q: What is the purpose of Durham test ?

A: In the carbohydrate fermentation test, we determine whether the bacteria are able to ferment a…

Q: To measure the culture temperature, why is the thermometer placed in a tube separate from the…

A: A microbe or microorganism is a microscopic organism that is too small to be seen with the naked…

Q: Aside from Paraffin, what is your recommended infiltrating medium for routine purposes? why?

A: INFILTRATION is the process in which the clearing agent is completely removed from the tissue and…

Q: Why is egg enrichment not added to BPA prior to sterilization?

A: Baird–parker agar or BPA is a selective medium is identify or isolate S. aureus in food. It contains…

Q: What products are sterilized using high energy ionizing radiation?

A: Sterilization is the process of killing microorganisms.

Q: Why is the temperature of pasteurization at 60°C?

A: Pasteurization was invented by a French Scientist called Louis Pasteur. It was first used in 1864.…

Q: which kind of sample gives positive result for Molish test?

A: It is a chemical test which is named after the botanist Hans Molisch.  This test involves the…

Q: Why should be avoided boiling more than 5 minutes in Barfoid test?

A: Barfoed test is carried out to detect the presence of monosaccharides in the sample. This test also…

Q: Why did we add agar after we measure the pH?

A: pH means p stands for potential or power.H stands for Hydrogen atom.It is the power of the Hydrogen…

Q: Which sample shows positive results in benedict's test? why?

A: Introduction: Benedict's test is a simple science test used to recognize reducing sugars.

Q: Why is sulfate (SO42-) added to a Winogradsky column?

A: Winogradsky developed the columns as a means of enriching sediments and soils for microbes.…

Physical and Chemical features

The physical property of a compound could be determined without modifying the originality of the compound. Examples of physical properties are density, hardness, color, boiling, and melting point. The chemical property expresses the ability of a compound to undergo a particular chemical change.