What is the concentration of a double stranded DNA sample with the following values? A260 - 1.068 A320 - 0.049
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Solved in 3 steps with 3 images
- Make 2 mL of 50 fold dilution of DNA solution and sodium phosphate buffer. DNA: 400 uL Sodium phosphate buffer, pH 6.9, 10mL - Calclate amount of DNA & buffer need to make dilutionIf the purity of DNA sample is below 1.8 A260/A280, where did the protein contamination come from?Why should the extracted DNA be immersed in TE buffer?
- Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.How does the pH of the buffer affect the sample? What are the implications on the separation of DNA fragments?Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE Buffer
- A DNA sample contains 21% adenine. What is the complete percentage base composition?What is the 260/280 ratio when quantifying DNA? select the correct option below A. ratio of protein to impurities in sample B. ratio DNA to impurties in sample C. ratio of DNA to protein in sample D. ratio of DNA to RNA in sampleDoes each sample contain pure DNA? How do you know?