What is the description of the growth and growth pattern of the microorganisms in the spread plate method? Type of Growth: Surface or Sub-surface? What is the typical growth pattern of inocula?
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What is the description of the growth and growth pattern of the microorganisms in the spread plate method?
Type of Growth: Surface or Sub-surface?
What is the typical growth pattern of inocula?
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- what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?What method of bacterial measurement does this graph represent? Is it considered direct, or indirect? What phase of the growth curve can NOT be measured using this method?After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold
- why is it important that a small amount of inoculum be used in preparation of an agar slant?Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial cultureWhy is 15 minutes generally used for sterilization? Why is agar added to medium?
- Does agar concentration affect the growth and morphology of bacterial colonies? What causes dense growth and what causes minimal growth?What is the most difficult type of microbial cell or structure to kill? What methods can be used to sterilize a sample contaminated with these?What is culture media? Which culture media is used for gram negative bacteria and which one for gram positive. Also explain strercobilinogen and corpophyrin?