After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?

After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.5: Tests For Group A Streptococcus

Problem 3RQ

See similar textbooks

Related questions

Q: Which arrestin is required for activation of phosphorylation of MDM2 during chronic Iso stimulation?…

A: A family of proteins known as arrestins is crucial for controlling cellular reactions and G…

Q: The TRP operon is regulated by two mechanisms, the TrpR regulatory protein and attenuation.…

A: The TRP operon is a well-studied genetic system that plays a crucial role in regulating the…

Q: For a long time, it was hypothesized that glycogen synthase employed an SN2 reaction mechanism via…

A: The mechanism by which glycogen synthase includes glucose units to a developing glycogen granule has…

Q: Consider a situation where the electrical potential inside a cell is 173 mV, the electrical…

Q: I need help with biology homework I’m stuck It asks you will have five carbohydrates kalose,…

A: In this question, we are presented with five carbohydrates: kalose, kaelose, ketrose, kalpose, and…

Q: Why is the mitochondria so important to a eukaryoWhich is an example of a prokaryotic cell?tic cell?…

A: The scientific investigation of cell structure and function is known as cell biology, and it is…

Q: Provide briefly the history of the two scientists behind understanding of genetics and describes the…

A: The field of genetics has a rich history of scientific inquiry and discovery, with notable…

Q: I need to see a line graph for the following data and which (if any) of the individuals (A or B) has…

A: draw a graph with x axis consist of time in hours and y axis labelling with glucose content in ml…

Q: please provide the structure of the enzyme's catalytic serine residue (as a monomer)

A: The catalytic serine residue in enzymes plays a crucial role in many biochemical reactions. The…

Q: A type of tomato (Solanum lycopersicum) produces fruit in three possible colors: red, green, and…

A: Epistasis is a genetic phenomenon where the expression of one gene masks or modifies the expression…

Q: Vascular endothelial growth factor receptor 2 (VEGFR-2) is an endothelial cell-specific receptor…

A: For the quantification of kinase activity, homogeneous time-resolved fluorescence resonance energy…

Q: You are tasked with cultivating the following microorganisms: A recombinant strain of E. coli A wild…

A: For E. coli recombinant strain: Complex Medium is chosen, for eg: Luria-Bertani (LB) broth Nutrient…

Q: What are the three function of Meiosis cell division? What is the end result of meiosis cell…

A: Meiosis is a fundamental process of cell division that plays a crucial role in sexual reproduction…

Q: 4. Illustrate (draw) what an action potential might look like with the following voltage-gated…

A: In this investigation, we look at how a few voltage-gated channels influence the appearance and…

Q: 25 year old male motorcyclist was brought into the emergency department following an accident. The…

A: The drop in blood pressure and increase in heart rate observed in the 25-year-old male motorcyclist…

Q: Give only typing answer with explanation and conclusion Is the first division after the egg is…

A: Fertilization is the process of permanent fusion of haploid (n) male and female gametes that results…

Q: In lecture we discussed how a measurement of linkage disequilibrium (D- the disequilibrium…

A: For four distinct recombination frequencies, we are investigating the decay of linkage…

Q: 17. Mitochondrial complexes I, III, and IV can all be considered to carry out an exergonic (energy-…

A: The bioenergetic processes carried out in the mitochondria are central to cellular metabolism and…

Q: repared by dissolving 0.171 mol of formic acid (HCOOH, K, -1.77x104) and 0.450 mol of hydrocyanic…

A: Answer.) :- To calculate the pH of the solution, we need to determine the concentration of the…

Q: Below are statements about symbiosis. Choose the correct responses. ( Only pick one answer per [ ]…

A: In nature animals, plants and microbes do not and cannot live in isolation but interact in various…

Q: i. The distal tubule has fewer pumps. ii. The descending arm of the loop of Henle is impermeable to…

A: The nephron is a fundamental structural and functional unit of the kidney responsible for filtering…

Q: 1. What techniques are used in the panel A,B C in figure 1? 2. What does the data show?

A: The provided figure, Figure 1, presents experimental observations related to gene expression errors…

Q: Characters Sponge Jelly X X Cells with flagella Bilateral symmetry Mesoderm Head develops first Anus…

A: The system of naming and categorising animals is known as taxonomy. The study of links between…

Q: Identify whether each of the following descriptions applies to typical prokaryotic genomes only,…

A: In these questions, we will learn about different things in cells, like their DNA (the instructions…

Q: Match the label to the correct structure on the chloroplast. Intermembrane space Stroma Thylakoid…

A: A chloroplast is a specialized organelle found in the cells of plants, algae, and some protists. It…

Q: List the components (in order of flow) of the: 1. Nephron 2. Renal blood vessels 3. Juxtaglomerular…

A: Understanding the urinary system and its components is crucial for grasping the principles of renal…

Q: 10. Nondisjunction of one chromosome at meiosis I in the mother can give rise to which of the…

A: Nondisjunction of one chromosome at meiosis I in the mother can give rise to the following…

Q: Suppose two parents, a father with the genotype AaBbccDd and a mother with the genotype aaBbCcDd,…

A: An organism's genotype, or the collection of alleles it contains for a certain trait, is referred to…

Q: Many phospholipase C enzymes contain pleckstrin homology (PH) domains. What is the function of…

A: Pleckstrin homology (PH) spaces are commonly found in numerous phospholipase C (PLC) enzymes. These…

Q: 5 1 2 10 3 4

A: Autosomal recessive inheritance refers to a pattern of inheritance where a trait or disorder is…

Q: Given your understanding of life histories and population dynamics, propose some ideas for how…

A: On the basis of their life cycles and population structure, diverse organism types can react to…

Q: I need help pleae

A: In cell #1 (permeable to cation A): Since the equilibrium potential for cation A in cell #1 is a…

Q: Answer the following question: - Compare and contrast the evolution of genetic diversity with the…

A: The evolution of genetic diversity within the human population can be compared and contrasted with…

Q: What conditions favor semelparity?

A: Understanding the factors that influence reproductive strategies in organisms is essential for…

Q: 13. Which of the following is correct with regards to temperature and genomes? A. The higher the…

A: The genome of an organism is made up of usually DNA. DNA is made up of nucleotides that consists of…

Q: What is WebMD authority

A: WebMD is a popular online health information platform that provides medical information, resources,…

Q: Describe a time when someone that experienced some sort of injury

A: Every year, millions of individuals worldwide engage in various sports and recreational activities.…

Q: Vascular endothelial growth factor receptor 2 (VEGFR-2) is an endothelial cell-specific receptor…

A: Angiogenesis, the formation of new blood vessels from pre-existing ones, plays a critical role in…

Q: A certain species of bacterium was grown with either glucose or sucrose as a carbon source. After a…

A: A bacterium's growth rate is the pace at which its population of bacterial cells expands over time.…

Q: Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map…

A: Horizontal gene transfer (HGT) is the exchange of genetic information across organisms, which…

Q: I II IV 2 2 2 3 3 2 3 4 5 6 5 6 7 7 5 8 2a) Identify the pattern of Mendelian inheritance…

A: Gregor Mendel is regarded as the father of genetics because of the work he did upon pea plants and…

Q: ake two coins and assume that heads represent the dominant allele (A) and tails represents the…

A: In this experiment, we are simulating genetic inheritance using two coins. We assume that heads…

Q: The Sal-like 1 gene (Sall1) plays a role in kidney development. Point mutations in the gene result…

A: In genetic engineering experiments, the antibiotic neomycin is frequently utilized as a selectable…

Q: W The graph below shows the maximum number of bacterial cells that can grow in a constant volume of…

A: Bacterial growth is the term used to describe the gradual rise in the quantity of bacterial cells,…

Q: You carry two alleles for every trait. Where did these traits come from?

A: Two types of nucleic acid are found in our cell, DNA (deoxyribo nucleic acid) and RNA (ribo nucleic…

Q: Before the technology existed to synthesize RNA molecules of defined sequence (like ACACACAC.....),…

A: To calculate the probability of finding a Proline codon (CCU, CCC, CCA, or CCG) in the random RNAs…

Q: Distinguish between and to explain the terms cells, tissues,organs and systems .

A: A complex organism with many interrelated systems, organs, tissues, and cells makes up the human…

Q: So when I was doing my biology homework I filled the chart and it was for the five carbohydrates…

A: When conducting carbohydrate tests using Benedict's reagent and iodine, we observed different…

Q: 1 What percentage of UTIs are caused by E. col? What other causative agents are associated with…

A: Urinary tract infections (UTIs) are one of the most common infections encountered. UTIs are more…

Q: Match each of the following with the best answer choice: B. the diffusion of water across a membrane…

A: Cell membrane transport refers to the movement of substances across the cell membrane, which…

Concept explainers

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.

Question

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1: TMTC:-

VIEW

Step 2: Causes of TMTC plates:

VIEW

Solution

VIEW

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold
Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agar
Are the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
Which isolation technique is best used in isolating microorganisms (in countable good plates) from a gram of soil sample with expectedly high microbial count? A. Streak Plate technique B. Serial Dilution-Pour Plate technique C. Spread Plate technique D. Hanging Drop technique 2. Which materials are needed in isolating bacteria using Streak Plate technique? Check all that apply. ( )nutrient agar plate ( ) inoculating loop ( ) microbial inoculum ( ) flame source 3. Which term refers to that solution of sterile distilled water/normal saline/peptone water used to "thin out" dense microbial populations in Serial Dilution-Pour Plate technique used in the isolation of microorganisms? A. inoculum B. culture medium C. diluent D. microbial culture
What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?
After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Choose ALL that apply) a - There is fungal growth in the original broth culture tube. b- There is too much growth on the agar slant. c- There are colonies of similar morphology on the slant. d - There are red, yellow, and white colonies on the slant. e - There is no growth on the slant.
What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
Does agar concentration affect the growth and morphology of bacterial colonies? What causes dense growth and what causes minimal growth?
What is the description of the growth and growth pattern of the microorganisms in the spread plate method?Type of Growth: Surface or Sub-surface?What is the typical growth pattern of inocula?
What is the most difficult type of microbial cell or structure to kill? What methods can be used to sterilize a sample contaminated with these?
In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
Is the Mueller-Hinton Agar (MHA) a complex or defined medium? Explain based on its composition. Is MHA a A) differential, B) selective, C) both differential and selective media, or D) neither? Explain based on what kind of microorganisms it allows to grow.