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What is the meaning of the different values given engraved on each objective?
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Solved in 3 steps
- How many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.Explain briefly why the 100X objective lens of the light microscope is immersed in oil.
- a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?FOV measured at 100x total magnification is 400 micrometers. What is the FOV at 40X total magnification? 2. If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In micrometers?if 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved?
- Assume you are observing the diatom pictured in Figure 1 using the 10X lens in a compound light microscope. You move to the 40X lens and then again to the 100X lens by only rotating the turret (remember that the lenses are parfocal), without making any other adjustments to the microscope. c) After making your adjustments, you notice that the midline of the diatom is in focus while the remainder is blurry. Explain, based on microscopy principles, why this has occurred Provide a description of the procedure to prepare an acidic stain of bacteria using Nigrosin as it would appear in the Methods section.Compare the two (or more) quantum dots emission at850 pbs/cds.. lnGaAs materials - which would be preferable for labeling certain molecules/organelles in biological samples for fluorescence microscopy? What were your considerations for this choice?You have a microscope equipped with 10 x oculars and 4 objectives, which exhibit the following characteristics (M, NA): A=(4 x/0.13); B=(20 x/0.4); C=(20 x/0.7); D=(40 x/0.65) Tick the right answers: o Objectives B and C are equivalent. o Objective D will allow more detailed observation than objective C. o To see small structures, you should choose objective D rather than C. o Objective D has a lower resolution than objective C. o To observe large objects, it is better to use objective B rather than objective C
- If 5X instead of 10X oculars were used with the same objectives now on your microscope, what magnifications would be achieved? Explain your answer.when viewing an organism under the 40x objective lens, you estimate that 6 organims could fit across the DFV if they were laid end to end and 20 could fit if stacked side by side. What is the length and width of this organism in micra?You used the stage micrometers to measure the FOV for your microscope at 400x total magnification at 225 µm Use the formula Mag1 × FOV1 = Mag2 × FOV2 to calculate the FOV for your microscope at 1000x total magnification___________ 40x total magnification____________ Convert the 40x FOV to mm: _____________