You are given the following DNA fragment to sequence: 5'-GCTTAGCATC-3'. You first clone the fragment in bacterial cells to produce sufficient DNA for sequencing. You isolate the DNA from the bacterial cells and carry out the dideoxy-sequencing method. You then separate the products of the polymerization reactions by gel electrophoresis. Draw the bands that should appear on the gel from the four sequencing reactions.

You are given the following DNA fragment to sequence: 5'-GCTTAGCATC-3'. You first clone the fragment in bacterial cells to produce sufficient DNA for sequencing. You isolate the DNA from the bacterial cells and carry out the dideoxy-sequencing method. You then separate the products of the polymerization reactions by gel electrophoresis. Draw the bands that should appear on the gel from the four sequencing reactions.

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 6QP: Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel,...

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