Q: Identify the type of mutation that occurred to produce the abnormal hemoglobin. Explain why you…
A: Nonsense mutation: This is a condition in which a protein loses its function, resulting in sickness…
Q: Below is a sequence of DNA.…
A: Introduction :- Adenine (A), thymine (T), cytosine (C), and guanine (G) are four nucleic acid bases…
Q: What are the differences between a DNA library and acDNA library?
A: For the gene cloning method, the DNA is used, which is cut and ligated in the vector. This…
Q: What Is a DNA Library?
A: For constructing DNA libraries, the DNA is processed using restriction enzymes. The components of…
Q: List essential components required for the DNA sequencing reaction based on the Sanger method.…
A: DNA sequencing is the process of determining the sequence of nucleotides in a piece of DNA. In…
Q: In DNA extracting. What is the purpose of clear shampoo in the DNA extraction buffer?
A: DNA is extracted from different sources to analyze and study the DNA sequence and diseases caused…
Q: If the sequence of mRNA is 5'-AAUCGUACGGAUGCCGAAAUACCCAUUAGGGAUUGCAUAGCGAGCAACGGAC-3', what is the…
A: Protein is the final product of a gene that determine the phenotype of the organism. Protein…
Q: What is a DNA read?
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: Give the sequence on the opposite strand for ACGTAT, AGATCT, and ATGGTA (all read 5' to 3'). Are the…
A: Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA) are the two types of nucleic acids forming…
Q: Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?
A: Escherichia coli also known as E. coli is a gram negative, facultative anaerobic, rod shaped,…
Q: Obtain the self-dotplot of the following sequence to identify repeat region:…
A: There are various alignment tools available at EMBOSS. It is studied under the domain of…
Q: what is a palindromic sequence?
A: If a nucleic acid sequence in a double stranded DNA or RNA molecule is read in a particular…
Q: a BLASTP
A: Introduction The Basic Local Alignment Search Tool (BLAST) observes districts of nearby similitude…
Q: Defi ne palindrome and draw three different palindromic sequences.
A: DNA( deoxyribonucleic acid) is the double-stranded molecule that is the genetic material in most…
Q: What does SDS–PAGE stand for? What is the benefit of doing SDS– PAGE?
A: Electrophoresis is the migration of charged particles under the influence of an electric current.…
Q: What does 'biological sequence database' refer to?
A: Bioinformatics aids in the storage and process of large biological information by the aid of…
Q: What is reading frame?
A: DNA stands for deoxyribonucleic acid. It is a genetic material that passes from parents to…
Q: Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in…
A: The polymerase chain reaction is the method that is used for making copies of DNA, it is developed…
Q: List and Describe the algorithm of BLOSUM substitution matrix computation.
A: The BLOSUM matrix is another amino acid substitution matrix, first determined by Henikoff. For its…
Q: Use the sequence alignment tool Smith and Waterman for the amino acid number 16-30 of the protein…
A: The Smith-Waterman algorithm uses local sequence alignment to find similar regions between two…
Q: What is SDS PAGE?
A: Electrophoresis is the migration of charged particles under the influence of an electric current.…
Q: What is an open reading frame?
A: A gene is a DNA-based functional heredity unit that delivers instructions for the production of RNA…
Q: Method on the following 5 sequences and find the best multiple sequence alignments. Sequence # 1:…
A: Progressive alignment is a heuristic for multiple sequence alignment that does not optimize any…
Q: What is meant by the phrase reading frame?
A: The genetic sequence is comprised of nucleotides, which are joined together to form polynucleotides…
Q: What is a read (Sanger sequencing)?
A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…
Q: John wants to build a 3D structure of his unknown sequence what steps he will perform discuss all…
A: The protein is studied as primary, secondary, tertiary, and quaternary structure. It is studied…
Q: What are the advantages and disadvantages of using indented and bracket type dichotomous key? What…
A: Key are the vital and inseparable part of taxonomy that help to identify the unknown species and…
Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…
A: In manual Sanger sequencing, four PCR reaction are set up, each with only a single type of ddNTP…
Q: Consider the following wild-type and mutant sequences:Wild-type ....CTTGCAAGCGAATC....Mutant…
A: Introduction A mutation occurs when the sequence of DNA changes. Mutations can occur as a result of…
Q: What is a DNA library? Do you think this name is appropriate?
A: In terms of molecular biology, a library can be defined as the collection of DNA fragments that are…
Q: Use a drawing to illustrate the principle of DNA gel electrophoresis. Indicate roughly the…
A: Gel electrophoresis technique is used to separate DNA, RNA and proteins depending on their molecular…
Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…
A: Sanger sequencing is a method of DNA sequencing based on the selective incorporation of…
Q: Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the…
A: Edman degradation, which was invented by Pehr Edman, can be used to determine the amino acid…
Q: Why evenly spaced sequence in chromatogram is an indication of a good DNA chromatogram?
A: The characteristics of an evenly spaced sequence are:- Evenly-spaced peak, each being…
Q: What defines an open reading frame?
A: Molecular genetics concerns with the chemical nature of the gene itself such as encoding of genetic…
Q: What is a Sanger Sequencing ?
A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…
Q: Explain how Sanger sequencing works
A: Genome sequencing: genome means total gene content present in the organism and sequence means to…
Q: Are the following base sequences sticky or not sticky? Each piece is written 5′ to 3′.(a) TTAGC and…
A: A base is nitrogen containing heterocyclic compound which, is found in DNA and RNA. There are…
Q: What is the primary disadvantage of Sanger sequencing?
A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…
Q: Based on the sequence data below. Which of the indicated sections of the sequence can you trust to…
A: DNA (Deoxyribonucleic acid) sequencing is an essential component of molecular biological studies.…
Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…
A: This method makes use of the mechanism of DNA synthesis by DNA polymerase DNA polymerase requires…
Q: What did Rosalind Franklin do when she saw Watson and Crick’s first model? Why?
A: After seeing Franklin's x-ray images of DNA and her report on DNA's symmetry, Watson and Crick built…
Q: What are some key points, based on your work, in constructing a dichotomous key?
A: The process applied by the students and professions to identify a substance like specimen, animals…
Q: What is the term ‘Eidetic image' refers to?
A: In the literature of science, the term eidetic image comes close to photographic memory.
Q: Describe Shine Dalgarno sequences
A: The process of translation is formation of a polypeptide chain from an mRNA sequence. This requires…
Q: What is the E-value of the sequence, what does your E-value reflect and what’s the importance of an…
A: Bioinformatics is the branch of science that deals with the genome, gene, and protein sequences. It…
Q: On the gel diagram below, show how you believe these fragments will sort out during electrophoresis.…
A: DNA is a double stranded helical molecule that is made up of repeating nucleotides. The nucleotides…
What is the symbol on the first line of a raw read that tells you a unique sequence follows?
Step by step
Solved in 2 steps
- How does what you see on the acrylamide gel reflect what is presented on a sequencing chromatograph?Where do you expect to see multiple “Ns” within a sequencing read? a)Within the first 25 nucleotides from the beginning of the sequence and from the last 25 nucleotides of the sequence. b)In the middle of the sequence and from the last 25 nucleotides of the sequence. d c)From the last 25 nucleotides of the sequence. d)Within the first 25 nucleotides from the beginning of the sequence. e)In the middle of the sequence.How would I calculate sanger sequencing fragments that will produce green
- The plot shows a correlation between sequence error rate and cluster density on an Illumina flow cell. From your knowledge of how Illumina sequencing works, why do you think there is this correlation?What are the advantages and disadvantages of using indented and bracket type dichotomous key? What are some key points, based on your work, in constructing a dichotomous key?Perform Progressive Alignment Method on the following 5 sequences and findthe best multiple sequence alignments.Sequence # 1: ATCCAATTTTSequence # 2: ACTGACCSequence # 3: ATGGCCATTSequence # 4: ATCTTCTTSequence # 5: ATTGCCATT