What nutrients do the following media components contain? • Peptone Yeast extract Beef extract • Potato infusion • Agar

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter6: Biomedical Electrodes, Sensors, And Transducers
Section: Chapter Questions
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What nutrients do the following media components contain?
• Peptone
• Yeast extract
• Beef extract
Potato infusion
• Agar
Why should agar media be completely dissolved before they are dispensed in tubes and
plates?
What are the bases for pegging the temperature at 121°C for 15-30 minutes during moist heat
sterilization and 180°C for two (2) hours using dry heat sterilization? 1. Can you sterilize
culture media using dry heat sterilization? Why is that so?
You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in
media prep, sterilization and culture of microorganisms?
Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it
technically acceptable in microbiology?
Transcribed Image Text:What nutrients do the following media components contain? • Peptone • Yeast extract • Beef extract Potato infusion • Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 121°C for 15-30 minutes during moist heat sterilization and 180°C for two (2) hours using dry heat sterilization? 1. Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?
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