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- What nutrients do the following media components contain? Peptone Yeast extract Beef extract Potato infusion Agar Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? 1.Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?Please include sourceMannitol salt agar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect mannitol fermentation. Which component of mannitol salt agar makes it a selective medium?Why would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?
- how do microorganisms such as bacteria eliminate harmful reactive oxygen species? Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…Agar is a solidifying agent used in media preparation.a. What is its origin? b. What makes it ideal for cultivation of microbes? c. How and why does the agar concentration in semisolid media differ from conventional solid media?
- Mannitol salt agar (MSA) only allows the growth of halophiles. Among the halophiles, mannitol fermenters release acid that turns the pH indicator yellow; mannitol non-fermenters leave the medium red. Onto MSA you inoculate a halophilic mannitol non-fermenter and a non-halophilic mannitol non-fermenter. What are your expected results in this experiment? Explain the role of the media in this case.The principle and What type pf Media of the following Conkey Agar vii) MacConkey Broth viii) TSA Base for Blood and chocolate Agar ix) Mannitol Salt Agar x) Tryptic Soy Agar xi) Alkaline peptone water What is the principle of Growth Medium and Culture medium?Microbial fermentation can be carried out as either a once off ----------------- culture or a-------------------------- culture. The correct terminology for these respective cultures & the vessel the latter is carried out in are? Select one: a. continuous, batch, fermenter b. batch, continuous, chemostat c. batch, continuous, turbidostat d. batch, continuous, turbidostat e. continuous, batch, chemostat
- What is biochemical oxygen demand (BOD) test? At what stage of Sewage treatment this test is performed? BOD level of three samples of water labelled as A, B and C are 30 mg/L, 10mg/L and 500 mg/L respectively. Which sample of water is most polluted?do methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar, LB-Dextrose Broth, Saline solution 0.85%, Glycerol 80%). Include photos or schematics.If you have a 15 mg/100 ml stock solution of GA3 and you need a 1 mg GA3 in 25 ml, how much stock solution would you add to 125 ml of medium? how to calculate these kind of question in tissue culture media preperation