What special precautions must be taken when using the oil immersion (100x) objective?

Q: What are the expected problems in the centrifuge in case of imbalance of samples ? *

A: Centrifugation is a process of separation of molecules from mixtures by applying centrifugal force…

Q: write the  protocol for Hexamine silver staining, including their reagents, materials, prinicple,…

A: Grocott’s alkaline hexamine-silver solution undergoes reduction to create precipitates of silver…

Q: List the steps that you implement while using oil immersion to observe the specimen.

A: An instrument that helps to see very small objects is known as a microscope. There are different…

Q: How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?

A: Spectrophotometry is a method in which the absorption or transmission properties of a material is…

Q: Find the amount of dehydrated medium needed to prepare 15 BUTTS, 20 SLANTS, and 25 BUTT-SLANTS.

A: Slants, Butt-slants, and butts are different types of media prepared for bacterial growth. Slants…

Q: Write the materials,  reagents, Principle and  procedure for the Periodic acid Schiff technique

A: Answer: STAINING = It is the method to colorize the smear or transparent tissue or cells in order to…

Q: what is the importance of getting retention factor (rf) value in chromotography tools?

A: Rf also known as retention factor is used in thin layer chromatography (TLC) and high performance…

Q: eservatives cannot be used for direct + mount ?

A: The disease is a state of the body in which the physiological mechanisms do not function in a normal…

Q: Which type of radiation is most likely to be used for isotope scanning?(a) alpha(b) beta(c) gamma

A: A radionuclide scan is an imaging method that utilizes a little dose of a radioactive chemical…

Q: When using the oil immersion lens, what four procedures can be implemented to achieve the maximum…

A: Microscopes are useful for viewing objects that are too small to see clearly without magnification.…

Q: Strong Iodine Solution USP Formula: Iodine pellets ____ Potassium iodide ____ Purified water ____…

A: Pharmacopeia has a collection of medicinal preparations, dosage forms, information about the purity…

Q: tate the apparatus used view small samples

A: The world is full of organisms. Organisms have developed from minute single celled structures to…

Q: Why is it necessary to flame the top of the bottle before pouring the plate?

A: Aseptic techniques are used to prevent contamination from pathogens. These are used in clinics and…

Q: Which objective should always be in place at the beginning of observing a specimen? O A) low (4X) B)…

A: An objective lens is one of the lenses present in a compound microscope. The objective lens are…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: Explain the reason for the effect on mass spectroscopy method of diagnosis by culture conditions.

A: Mass Spectrometry is an analytical method. It is used to quantify known materials to identify…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…

A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…

Q: When using the 40X objective, the total magnification is

A: The total magnification of microscope must be calculated by multiplying the magnification of eye…

Q: Write down the materials , reagents ,instruments,  principle and procedure for Grocott’s hexamine…

A: The Grocott's hexamine silver stain is used to demonstrate fungi. It is a part of histopathology…

Q: Dropping Mercury Electrode

A:

Q: Outline the acid fast staining technique using a flow chart.

A: ACID FAST STAINING TECHNIQUE : it is the differential staining techniques which was first developed…

Q: What is the total magnification possible with a 10x objective?

A: The microscope is used to view the objects that are not seen through the naked eye. Microscopes use…

Q: n is 100x, what is the magnification of n is 40x, what is the magnification of t gnification is 10x,…

A: In light microscopy, there are several different types of microscopes, but the four most common are…

Q: What is the reason for the decrease in the ABS value over time when measuring in the…

A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…

Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?

A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…

Q: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

A: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

Q: The technique of bombarding specimens with neutronsand measuring the resultant gamma ray emissions…

A: Neutrons are the subatomic particles present in the nucleus of an atom.

Q: What is the importance of sllid width in fluorescence spectroscopy

A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.

Q: In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the…

A: A spectrophotometer is one of the most frequent tools used by scientists to determine the presence…

Q: True- False Acetone is the safest solvent for cleaning an objective lens.

A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…

Q: If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what…

A: Magnification is defined as a measure of the ability of a lens or other optical instruments to…

Q: How many grams of media would be required to prepare 350 plates assuming 20 mL per plate? O 72 O 112…

A: The preparation of  mentioned Escherichia coli culture media contain 36 grams per litre deionized…

Q: Write the protocol for lactophenol blue stain method indicating; materials,  reagents,  Principle,…

A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…

Q: Total magnification of a specimen using the high objective and the ocular lens is

A: B) 40Ox 400 x is the typical magnification of an ocular lens.

Q: Why do you use oil on the slide to be examined with the oil-immersion objective?

A: Oil immersion microscopy increases the refractive index of a specimen when used in the correct way.…

Q: What steps are required to verify both contact with the sterilant and sterilization of an instrument…

A: Sterilization is the process that eliminates, removes, kills or deactivates the microorganism such…

Q: RODAC plates are used for Select one: Water sampling Air sampling Sterility testing Surface…

A:

Q: What is the hazard of the spattering tendency in heating

A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…

Q: Write down the materials, reagents, Principle, procedure  and results for Ziehl-Neelsen stain

A: It is a differential staining technique which was first developed by Ziehl and later on modified by…

Q: Give a vivid explanation of the definition, principles, types or forms, standard operating procedure…

A: Antigens are defined as the substances or the molecules that are present on the surface of the…

Q: Alternative name for Moist-heat sterilization Select one: Oven sterilization Autoclaving Ionizing…

A: Sterilization is any technique that eliminates, removes, or disengages every living organism…

Q: why is it necessary to use oil immersion on oil objective ?

A: The oil immersion is the technique of light microscopy, which is used to increase the resolving…

Q: What is the stationary and mobile phase of spectrophotometry?

A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…

Q: What are the immersion media that need to be used?

A: Immersion media is used to increase the numerical aperture of the microscope by a factor equal to…

Q: In RPR test, Why is it necessary to rotate the slide/card?

A: RPR stands for Rapid Plasma Reagin. It is done to detect syphilis.

Q: Why must the entire calibration procedure be performed for each objective?

A: An ocular micrometer has a circular "glass disk" that fits in a microscope eyepiece and possesses a…

Q: What is the purpose of examining your specimen slide using low power objective first nefore high…

A: Objective lens are magnifying lens that functions to magnify an image. These lens make an important…