Outline the acid fast staining technique using a flow chart.

Q: Define the sub coating technique

A: Tablets solid formulations that undergo several modifications in the body. So, to decrease the loss…

Q: is it essential that the primary stain and counterstain be of contrasting colors

A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…

Q: Describe the Gram staining procedure

A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria.  These…

Q: Give two reasons for heating the slide after the smear is air dried?

A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…

Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…

A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…

Q: Sample diagram for the gram staining procedure and microscopic observation.

A: Danish Bacteriologist Hans Christian Gram in 1884 introduced differential staining techniques in…

Q: Differentiate between negative and positive staining, givingexamples.

A: These are the staining methods that are used to see bacteria under a microscope.

Q: List the various staining methods, and briefly characterize each.

A: Regardless of the ability of the microscope to magnify and resolve, staining is necessary to create…

Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.

A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…

Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…

A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…

Q: Complete the table below. For the function, identify the reagent if it is a stain, clearing agent,…

A: Answer : The function of reagents are :  1- FAA : fixative  2- ethyl alcohol : chemical solvent  3-…

Q: yield

A: The Dische's test is a chemical test that is utilized to detect the presence of DNA in a solution.It…

Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…

A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: How can polarography detect the analyte?

A: Jaroslav Heyrovsky discovered polarographic method   Polarography is a simple method in analytical…

Q: Write down the procedure for millipore filter technique

A: The Millipore filter technique is a new method for the concentration, removal, and enumeration of…

Q: Gram staining techniques

A:

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the…

A: The process of Gram Staining on any sample requires four basic steps that include applying a primary…

Q: Summarize the steps for general Hematoxylin and Eosin staining procedure through a flow chart.

A: Hematoxylin and eosin staining It is used to identify recognize different types of tissues and their…

Q: Give three situations where the negative staining procedure would be used.

A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…

Q: write the  protocol for mann's methyl blue-eosin staining, including their reagents, materials,…

A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…

Q: Differentiate the “hot method” and “cold method” of acid-fast staining

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: List the reagents of the Gram stain technique in order and their general role in the staining…

A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…

Q: Select all of the following that represent examples of positive stains. Check All That Apply…

A: Staining is the common laboratory procedure which can be specific and general according to the use.…

Q: Define the term gram staining.

A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…

Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: list the stains and decolorizing agent used in acid fast staining method.

A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…

Q: Regarding the Gram stain procedure: whether Gram-positive or Gram-negative, cells may appear…

A: Q. Regarding the Gram Strain procedure: Whether Gram positive or Gram negative,cells may appear…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: What special precautions must be taken when using the oil immersion (100x) objective?

A: Using oil immersion reduces the air gap between the glass slide and the objective lens. This air gap…

Q: Outline the main differential stains and how they are used.

A: The detection and observation of cells and microorganisms in their natural state under microscope is…

Q: List the step-by-step procedure of Gram staining.

A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…

Q: The most commonly employed chemical test to determine the presence of blood in stain found at the…

A: Answer is option E"Teichman's test". this test is also called by names haematin crystal acid test or…

Q: Write the protocol for lactophenol blue stain method indicating; materials,  reagents,  Principle,…

A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…

Q: Write the instructions, reagents, principles and the procedures for the following.  1. Periodic acid…

A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…

Q: the steps in doing gram staining using a flow chart.

A: Gram-staining is a process based on the ability of bacteria to retain crystal violet dye. The…

Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…

A: Polychromatic solution of Methyl Blue and Eosin Y stains (modified Mann's solution) is  used for…

Q: what are the reagents for the four major steps of the gram stain procedure

A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…

Q: Write down the materials, reagents, Principle, procedure  and results for Ziehl-Neelsen stain

A: It is a differential staining technique which was first developed by Ziehl and later on modified by…

Q: differential staining procedures

A: Differential staining :  Differential staining is one of the important staining method. In this…

Q: stain

A: Cartilage is a smooth elastic tissue, it protects the bones at the joint by padding it.

Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…

A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…

Q: What are the immersion media that need to be used?

A: Immersion media is used to increase the numerical aperture of the microscope by a factor equal to…

Q: How can the thickness of the smear affect the staining procedure?

A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…

Q: cold method of Acid-fast staining.

A:

Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…

A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…