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- German measles results from an infection of the rubella virus, which can cause a multitude of health problems in newborns. What conclusions can you reach from a nucleic acid analysis of the virus that reveals an A + G/U + C ratio of 1.13?RNA was extracted from certain virus and found to contain 35% cytosine. With this information, is it possible to predict what percentage of the bases in virus are adenine? If so, what percentage? If not, why not?A virus has a double-stranded RNA genome that has 2400 base-pairs. Of these, 35 % are G:C base-pairs. How many uracil nucleotides would be present in this virus?
- Heinz Shuster collected the following data on the base composition of ribgrass mosaic virus (H. Shuster, in The Nucleic Acids: Chemistry and Biology, vol. 3, E. Chargaff and J. N. Davidson, Eds. New York: Academic Press, 1955). On the basis of this information, is the hereditary information of the ribgrass mosaic virus RNA or DNA? Is it likely to be single stranded or double stranded? Percentage A G C T U Ribgrass mosaic virus 29.3 25.8 18.0 0.0 27.0The following are DNA fragments containing a small gene. The top strand is the coding strand. Transcribe all 5 groups and translate. Group A 5’-GGCAATGGGTTTGTGCAATTCTAAAAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACGTTAAGATTTTCAAAAATTAAG-5’ Group B 5’-GGCAATGGGTTTGTGAAATTCTAAAAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACTTTAAGATTTTCAAAAATTAAG-5’ Group C 5’-GGCAATGGGTTTGTGCAATTCTAAGAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACGTTAAGATTCTCAAAAATTAAG-5’ Group D 5’-GGCAATGGGTTTGTGCAATTCTAACAGTTTTTAATTC-3’ 3’-CCGTTACCCAAACACGTTAAGATTGTCAAAAATTAAG-5’ Group E 5’-GGCAATGGGTTTTGCAATTCTAAAAGTTTTTAATTC-3’ 3’-CCGTTACCCAAAACGTTAAGATTTTCAAAAATTAAGYou are studying RNA viruses and have discovered a new one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don’t know if it is plus or minus stranded. Your lab-mate says, “Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome.” You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate’s suggestion?
- The base composition of an RNA virus was analyzed and found to be 14.1% A, 14.0% U, 36.2% G, and 35.7% C. Would you conclude that the viral genetic material is single-stranded RNA or doublestranded RNA?Heinz Shuster collected the following data on the base composition of ribgrass mosaic virus (H. Shuster, in The Nucleic Acids: Chemistry andBiology, vol. 3, E. Chargaff and J. N. Davidson, Eds. New York: Academic Press, 1955). On the basis of this information, is the hereditary information of the ribgrass mosaic virus RNA or DNA? Is it likely to be single stranded or double stranded?A virus has a double-stranded DNA genome that has 4600 base-pairs. Of these, 54 % are A:T base-pairs. How many cytosine nucleotides would be present in this virus? Show all your calculations.
- You are studying RNA viruses and have discovered one that grows well in a culture of eukaryotic cells. You know that the virus is a single-stranded RNA virus, but you don't know if it is positive or negative stranded. Your lab-mate says, "Well, just treat your cell culture with cyclohexamide and see if the virus replicates its genome." You know that cyclohexamide inhibits protein elongation by binding to eukaryotic ribosomes. What is the basis of your lab-mate's suggestion?Imagine that a colleague asks you to align a conserved spike protein-coding gene sequence from SARS-CoV2 virus (Severe acute respiratory syndrome-related coronavirus variant 2) to its homolog (i.e., equivalent spike protein-coding gene sequence) in the MERS virus (Middle East respiratory syndrome-related coronavirus). Which sequence type - nucleic acid (DNA or RNA), or protein - would you expect to exhibit the highest percentage of identities? Why?The smallpox virus, which actually has a fairly large genome size for a virus (about 190,000 base pairs), must use which of the following enzymes to replicate its nucleic acid? A. DNA-dependent-DNA-polymerase B. RNA-dependent-DNA-polymerase C. DNA-dependent-RNA-polymerase D. RNA-dependent-RNA-polymerase E. reverse transcriptase