When the S.cerevisiae genome was sequenced and surveyed for possible genes, only about 40% of those genes had been previously identified in forward genetic screens.  This left about 60% of predcited genes with no known function, leading some to dub the genes fun (function unknown) genes. a)As an approach to understanding the function of a certain fun gene, you wish to create a loss of function allele.  How would you do this? b)You wish to know the physical location of the encoded protein product.  How would you obtain such information?

Human Anatomy & Physiology (11th Edition)
11th Edition
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When the S.cerevisiae genome was sequenced and surveyed for possible genes, only about 40% of those genes had been previously identified in forward genetic screens.  This left about 60% of predcited genes with no known function, leading some to dub the genes fun (function unknown) genes.

a)As an approach to understanding the function of a certain fun gene, you wish to create a loss of function allele.  How would you do this?

b)You wish to know the physical location of the encoded protein product.  How would you obtain such information?

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