In recombination studies of the rII locus in phage T4, what is the significance of the value determined by calculating phage growth in the K12 versus the B strains of E. coli following simultaneous infection in E. coli B? Which value is always greater?
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
In recombination studies of the rII locus in phage T4, what is the
significance of the value determined by calculating phage growth
in the K12 versus the B strains of E. coli following simultaneous
infection in E. coli B? Which value is always greater?
Seymour Benzer developed an experimental system around 1950s for analyzing various subparts of a gene by creating crosses between T4 bacteriophage strains. All these mutant strains are pathogens of E. coli. This system is called T4 rII system. The bacteriophage infected E. coli colonies would be identified by the characteristic plaque.
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