Which among the following demonstrates the difference between Native Polyacrylamide Gel Electrophoresis (PAGE) and SDS-PAGE? Group of answer choices Native PAGE has a denaturation step whereas SDS-PAGE does not have a denaturation step. Native PAGE is based on both the charge and size whereas SDS-PAGE is only based on size. Native PAGE does not have a denaturation step whereas SDS-PAGE has a denaturation step. Native PAGE is based only on charge whereas SDS-PAGE is based on both charge and size.

Which among the following demonstrates the difference between Native Polyacrylamide Gel Electrophoresis (PAGE) and SDS-PAGE? Group of answer choices Native PAGE has a denaturation step whereas SDS-PAGE does not have a denaturation step. Native PAGE is based on both the charge and size whereas SDS-PAGE is only based on size. Native PAGE does not have a denaturation step whereas SDS-PAGE has a denaturation step. Native PAGE is based only on charge whereas SDS-PAGE is based on both charge and size.

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter24: New Technologies And Advances In Medical Instrumentation

Section: Chapter Questions

Problem 2RT

See similar textbooks

Similar questions

In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.
In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
Define about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain importance of sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ?
Which of the following techniques takes advantage of NET CHARGE to purify/characterize proteins? a. Isoelectric focusing b. Hydrophobic chromatography c. Affinity chromatography d. DialysisExplain.
Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.
How do we ensure that gel electrophoresis is functional? a Running buffer, containing electrolytes, allows for electrical current. b The apparatus must be either vertical or upright. c The matrix of the gel must be set to the appropriate percentage. d All proteins need to be denatured and given a unique charge.
Which of the following correctly matches the chromatography technique with the molecular property being exploited for separation? ion-exchange: charge,affinity: polarity, gel-filtration: molecular mass ion-exchange: charge, affinity:molecular binding preference,gel-filtration: molecular size ion-exchange: charge,affinity: molecular binding preference,gel-filtration: isoilectric point ion-exchange: ion binding preference,affinity: molecular size,gel-filtration:polarity
Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.
A student carries out the following experiments and records the following observations. 1. A few drops of a solution of KOH (aq) is added to a solution of Fe(NO3)3 (aq). A red gelatinous solid forms immediately upon addition. 2. A few drops of a solution of KOH (aq) is added to a solution of NaNO3 (aq). No changes are observed. 3. A few drops of a solution of KOH (aq) is added to a solution that could contain Fe3+(aq) and/or Na+(aq) ions (the solution could contain both ions, just one ion, or neither ions). A red gelatinous solid forms immediately upon addition. Select all of the answers that are consistent with the data from those 3 experiments. Group of answer choices The unknown contains Fe3+(aq) and Na+(aq) The unknown contains Fe3+(aq) It is not possible to determine the presence of Na+(aq) from this experiment The unknown contains neither Fe3+(aq) or Na+(aq)
Place the following steps for setting up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel running apparatus . I. Add Sybr-safe to the molten agarose. J. Connect the leads to both the gel running apparatus and the power pack .
The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.
Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the immobilised pH gradient (IPG) strip with pH range of 4-7. After the electrophoresis and staining, only ONE band is observed on the middle of the IPG strip. The band is a protein band. Briefly explain why only the protein band and NOT the DNA band appear on the IPG strip.