up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel run

up an agarose gel in order : A. Let the gel solidify B. Turn on the current and run gel . C. Pour the molten agarose into the gel cast . D. Load ladder and DNA samples into the well . E. Assemble the gel cast including comb . F. Place lid on the gel running apparatus . G. Remove the comb and add running buffer. H. Orient the gel in the gel run

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 2Q

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Choose the one answer that fits best. Which of the following statements regarding loading the well of a gel during electrophoresis is NOT correct? a. Holding the tip far above the well will make sure your solution sinks into the well properly b. Depressing the plunger too fast or too far can cause air bubbles in the well c. Depressing the plunger too fast while in the well may cause your solution to squirt out of the well d. Inserting the tip too far into the well may poke a hole into the well bottom e. You should always add glycerol to your supernatant to help your solution settle at the bottom of the well
Which of the following is NOT a function of the loading buffer used in SDS-PAGE? A) It contains a dye, letting you track the progression of electrophoresis samples B) It keeps the sample from floating away out of the gel well C) It measures the protein concentration of your samples D) It denatures proteins to turn them into linear chains of amino acids
Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.
In the assay of citric acid, what do you think about the nature of solution sample ? a) Before titration and b) After titration.
Which among the following demonstrates the difference between Native Polyacrylamide Gel Electrophoresis (PAGE) and SDS-PAGE? Group of answer choices Native PAGE has a denaturation step whereas SDS-PAGE does not have a denaturation step. Native PAGE is based on both the charge and size whereas SDS-PAGE is only based on size. Native PAGE does not have a denaturation step whereas SDS-PAGE has a denaturation step. Native PAGE is based only on charge whereas SDS-PAGE is based on both charge and size.
If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.
What are the things you need to consider when doing the following: a. Preparing/determining the concentration of the agarose gel- b. Loading the samples- c. Choosing the stain- d. Placing the electrodes on the power source- Briefly describe the function/s of each of the following: e. Gel f. Comb g. Stain h. Running buffer j. Molecular weight ladder/DNA size standard k. Micropipette l. Tracking dye m. Anode (red) n. Cathode (black) o. Microwave oven
What are the different applications of Gel Electrophoresis? explain each applications
How do we ensure that gel electrophoresis is functional? a Running buffer, containing electrolytes, allows for electrical current. b The apparatus must be either vertical or upright. c The matrix of the gel must be set to the appropriate percentage. d All proteins need to be denatured and given a unique charge.
Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.
In a polyacrylamide gel electrophoresis, the gel consists of specific gel components. name them
When loading samples onto a gel, why should expelling all the liquid in the tip be avoided? a)All the sample could be forced out from the well in which it was loaded. b)The extra liquid would end up in the buffer and this would distort the migration of the sample. c)The gel could tear and distort the migration of the sample. d)An air bubble could result which would distort the migration of the sample .