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- Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?Antibiotic X binds to the 50S ribosomal subunit of 70S ribosomes and blocks normal ribosomal function. Which of the following is true of this drug? It would interfere with protein synthesis in bacteria, but not affect eukaryotic ribosomes. It would interfere with peptidoglycan syntheis/repair in bacteria, but not affect eukaryotes as they lack peptidoglycan. It would interfere with mRNA transcription in bacteria and in eukaryotes. It would interfere with protein synthesis in bacteria and eukaryotes. It would interfere with mRNA transcription in bacteria, but not affect eukaryotic transcription. It would interfere with peptidoglycan syntheis/repair in bacteria, as well as in eukaryotes that possess cell walls.link: https://www.aaas.org/news/science-newly-identified-bacteria-break-down-tough-plastic How is the new PETase (2018) different from the 2016 version in function?
- Are there specific E. coli strains used to make competent cells? Explain the characteristics of these strainsYou are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?TRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
- Part A What kind of inhibition is imposed on HIV protease by ritonavir? O irreversible inhibition O reversible inhibition O uncompetitive inhibition. Submit Request AnswerMatch the statement with the correct term Statement- -A group of HFr bacteria is mixed with a group of F– bacteria. After several days, the F- bacteria contain part of the Hfr chromosome -The uptake of DNA into a bacterium from its environment -Requires the presence of a plasmid -uses bacteriophage to provide new genetic information Terms- -transduction -conjugation -translation -transformation -transpositionMany microbes (as well as other organisms) produce antibiotics. A natural compound produced by one organism that inhibits or kills another unrelated organism is commonly defined as an antibiotic. Thousands of antibiotics have been identified and characterized. Antibiotics prevent virtually every step in the Central Dogma of bacteria. Rifampicin, for example, inhibits transcription while streptomycin inhibits translation. However, no antibiotic has ever been discovered that directly affects DNA replication by inhibiting the action of DNA polymerases. "Why not?"
- you plated E. coli that had been transformed with your ligation reactions. There will also be a positive control that has E coli transformed with PHSG298 and a negative control that had E. coli transformed with water. Each was plated on both LB plates and LB + kanamycin plates. Match the growth pattern you expect for each transformation and each plate below for growth or no growth a. Negative control transformation plated on LB + kanamycin plates b. Ligation Transformation plated on LB + kanamycin c. Positive control transformation plated on LB + kanamycin plates d. Ligation transformation plated on LB e. Negative control transformation plated on LB plates f. Positive control transformation plated on LBThe human intestinal tract is home to a vast, diverse microbial community. The bacteria that comprise this microbial community serve a number of important, beneficial functions. However, some bacterial species have been suggested to play a role in certain forms of cancer. One such bacteria is Fusobacterium nucleatum (i.e., F. nucleatum). Investigators wishing to study the role of F. nucleatum in colorectal cancer developed a new assay for detecting these bacteria in tumor tissue. In controlled experiments, the test was highly sensitive in detecting F. nucleatum and also had high specificity. To examine consistency in their test findings, the investigators also conducted repeat tests in 50 tissue samples. From these repeat tests, the investigators observed that the overall number of samples in which F. nucleatum was detected was almost identical (10 out of 50 on the first test vs. 11 out of 50 on the repeat test); for 9 samples, F. nucleatum was detected on both the initial and the…Which of the following prokaryotic cells would probably not be “competent” at transformation? an Escherichia coli cell blasted with a “gene-gun” carrying DNA on gold particles an Escherichia coli cell treated with dimethyl-sulfoxide (DMSO) a Streptococcus pneumoniae cell with no special treatments an Escherichia coli cell with no special treatments E. an Escherichia coli cell treated with calcium