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- A positive lipid hydrolysis test is indicated by the growth of bacteria on the medium. A positive lipid hydrolysis test is indicated by the growth of bacteria on the medium. True FalseWhich of the following can most likely increase the yield of recrystallized benzoic acid? CHOICES: A fluted filter paper was used during cold filtration. The receiving flask from hot filtration does not contain a small amount of solvent. Activated charcoal was not added to the solution. The solution was filtered immediately after hot filtration.Nitrate reduction to nitrite is indicated by the formation of a pink to red colour after addition of nitrate test reagent. However, if a culture does not produce a colour change several posibilies exist. Explain.
- which of the following is best to sterilize heat-liable (sensitive) solutions? a, pasteurization b. autoclave c. dry heat d. filration e. none of the aboveSamples subjected to a test were given. Determine the reagent/material in the test. Methyl salicylate in Spot Test Beeswax in Saponification Test Linseed oil in Iodine Test Tallow in Acrolein Test Perla in Formation of Soluble Salt Cholesterol in Salkowski TestWhich of the following is used in the sterilization of the medium?a) benmargeb) swabc) autoclaved) centrifugee) cooling
- Given Test Reagent Positive or Negative Result Reason/Explanation Lime Soda Molisch Test Sucrose Fehling’s Test Fructose Tollen’s TestAll recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium…CITRATE UTILIZATION TEST WAS PERFORMED IN A CULTURE MEDIA WHICH CONTAIN A STRONG BUFFER. AND EVEN A SURE POSITIVE ORGANISM GET A NEGATIVE RESULT. WHY?
- Why should be avoided boiling more than 5 minutes in Barfoid test?What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeCreate a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.