All recipes are given per litre unless specified. Lambda Broth (LB) Plates Top Agar 10 g tryptone 10 g Tryptone 2.5 g NaCl 2.5 g NaCl 15 g Agar 7 g Agar Make 5 mL aliquots, then autoclave. Lambda Broth (LB) Media Suspension Media 10 g tryptone 5.0 g NaCl 2.5 g NaCl 2 g MgSO4· 7H2O 50 ml 1 M Tris pH 7.5 In LB medium The volume of each top agar is 5mL. The stock concentration of MgSO4 is 1M and must be diluted to ~10mM. What volume of stock MgSO4 will you add to each top agar?
All recipes are given per litre unless specified.
Lambda Broth (LB) Plates Top Agar
10 g tryptone 10 g Tryptone
2.5 g NaCl 2.5 g NaCl
15 g Agar 7 g Agar
Make 5 mL aliquots, then autoclave.
Lambda Broth (LB) Media Suspension Media
10 g tryptone 5.0 g NaCl
2.5 g NaCl 2 g MgSO4· 7H2O
50 ml 1 M Tris pH 7.5
In LB medium
The volume of each top agar is 5mL. The stock concentration of MgSO4 is 1M and must be diluted to ~10mM. What volume of stock MgSO4 will you add to each top agar?
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